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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hotta, Akitsu Cheung, Aaron Y. L. Farra, Natalie Vijayaragavan, Kausalia Séguin, Cheryle A. Draper, Jonathan S. Pasceri, Peter Maksakova, Irina A. Mager, Dixie L. Rossant, Janet Bhatia, Mickie Ellis, James |
| Description | Country affiliation: Canada Author Affiliation: Hotta A ( Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada.) |
| Abstract | Induced pluripotent stem (iPS) cells may be of use in regenerative medicine. However, the low efficiency of reprogramming is a major impediment to the generation of patient-specific iPS cell lines. Here we report the first selection system for the isolation of human iPS cells. We developed the EOS (Early Transposon promoter and Oct-4 (Pou5f1) and Sox2 enhancers) lentiviral vector to specifically express in mouse and human embryonic stem cells but not in primary fibroblasts. The bicistronic EOS vector marked emerging mouse and human iPS cell colonies with EGFP, and we used puromycin selection to aid the isolation of iPS cell lines that expressed endogenous pluripotency markers. These lines differentiated into cell types from all three germ layers. Reporter expression was extinguished upon differentiation and therefore monitored the residual pluripotent cells that form teratomas. Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| Issue Number | 5 |
| Volume Number | 6 |
| e-ISSN | 15487105 |
| Journal | Nature Methods |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2009-05-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Cell Dedifferentiation Genetics Cell Separation Methods Genes, Reporter Genetic Vectors Lentivirus Pluripotent Stem Cells Cytology Metabolism Animals Cell Differentiation Drug Effects Dna Transposable Elements Embryo, Mammalian Embryonic Stem Cells Enhancer Elements, Genetic Fibroblasts Green Fluorescent Proteins Humans Methyl-cpg-binding Protein 2 Mice Mice, Inbred Nod Mice, Mutant Strains Mice, Scid Promoter Regions, Genetic Rett Syndrome Pathology Teratoma Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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