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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Cambridge, Sidney B. Geissler, Daniel Calegari, Federico Anastassiadis, Konstantinos Hasan, Mazahir T. Stewart, A. Francis Huttner, Wieland B. Hagen, Volker Bonhoeffer, Tobias |
| Description | Country affiliation: Germany Author Affiliation: Cambridge SB ( Max Planck Institute of Neurobiology, Munich-Martinsried, Germany. cambridge@ana.uni-heidelberg.de) |
| Abstract | High spatial and temporal resolution of conditional gene expression is typically difficult to achieve in whole tissues or organisms. We synthesized two reversibly inhibited, photoactivatable ('caged') doxycycline derivatives with different membrane permeabilities for precise spatial and temporal light-controlled activation of transgenes based on the 'Tet-on' system. After incubation with caged doxycycline or caged cyanodoxycycline, we induced gene expression by local irradiation with UV light or by two-photon uncaging in diverse biological systems, including mouse organotypic brain cultures, developing mouse embryos and Xenopus laevis tadpoles. The amount of UV light needed for induction was harmless as we detected no signs of toxicity. This method allows high-resolution conditional transgene expression at different spatial scales, ranging from single cells to entire complex organisms. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| Issue Number | 7 |
| Volume Number | 6 |
| e-ISSN | 15487105 |
| Journal | Nature Methods |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2009-07-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Doxycycline Pharmacology Gene Expression Drug Effects Radiation Effects Animals Animals, Genetically Modified Analogs & Derivatives Chemistry Embryo Culture Techniques Embryonic Development Genetics Female Green Fluorescent Proteins Hippocampus Metabolism Larva Mice Mice, Transgenic Microscopy, Fluorescence, Multiphoton Photobiology Pregnancy Recombinant Proteins Tissue Culture Techniques Ultraviolet Rays Xenopus Laevis Growth & Development Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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