NDLI: Light-based feedback for controlling intracellular signaling dynamics.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Toettcher, Jared E. Gong, Delquin Lim, Wendell A. Weiner, Orion D.
Description	Country affiliation: United States Author Affiliation: Toettcher JE ( Cardiovascular Research Institute and Department of Biochemistry, University of California San Francisco, San Francisco, California, USA.)
Abstract	The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.1700
Journal	Nature Methods
Issue Number	10
Volume Number	8
Language	English
Publisher	Nature Publishing Group
Publisher Date	2011-09-11
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Feedback, Physiological Radiation Effects Signal Transduction Animals Cell Membrane Drug Effects Metabolism Computational Biology Models, Biological Phosphatidylinositol 3-kinases Antagonists & Inhibitors Chemistry Protein Binding Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Feedback loops shape cellular signals in space and time.

IGF-1-induced phosphorylation and altered distribution of TSC1/TSC2 in C2C12 myotubes

Strain-induced proliferation requires the phosphatidylinositol 3-kinase/AKT/glycogen synthase kinase pathway.

Inhibitors of phosphatidylinositol-3-kinase in cancer therapy.

Class II phosphoinositide 3-kinase defines a novel signaling pathway in cell migration

PI(3,4,5)P3 potentiates phospholipase C-beta activity.

Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism

Neurokinin 1 receptor mediates membrane blebbing in HEK293 cells through a Rho/Rho-associated coiled-coil kinase-dependent mechanism.

Cells see the light to bring signaling under control.

Light-based feedback for controlling intracellular signaling dynamics.

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