NDLI: A culture system to study oligodendrocyte myelination processes using engineered nanofibers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Seonok Leach, Michelle K. Redmond, Stephanie A. Chong, S. Y. Christin Mellon, Synthia H. Tuck, Samuel J. Feng, Zhang-Qi Corey, Joseph M. Chan, Jonah R.
Description	Country affiliation: United States Author Affiliation: Lee S ( Department of Neurology, University of California, San Francisco, San Francisco, California, USA.)
Abstract	Current methods for studying central nervous system myelination necessitate permissive axonal substrates conducive to myelin wrapping by oligodendrocytes. We have developed a neuron-free culture system in which electron-spun nanofibers of varying sizes substitute for axons as a substrate for oligodendrocyte myelination, thereby allowing manipulation of the biophysical elements of axonal-oligodendroglial interactions. To investigate axonal regulation of myelination, this system effectively uncouples the role of molecular (inductive) cues from that of biophysical properties of the axon. We use this method to uncover the causation and sufficiency of fiber diameter in the initiation of concentric wrapping by rat oligodendrocytes. We also show that oligodendrocyte precursor cells display sensitivity to the biophysical properties of fiber diameter and initiate membrane ensheathment before differentiation. The use of nanofiber scaffolds will enable screening for potential therapeutic agents that promote oligodendrocyte differentiation and myelination and will also provide valuable insight into the processes involved in remyelination.
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.2105
Journal	Nature Methods
Issue Number	9
Volume Number	9
Language	English
Publisher	Nature Publishing Group
Publisher Date	2012-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Cell Culture Techniques Myelin Sheath Physiology Nanofibers Chemistry Nanotechnology Oligodendroglia Cytology Animals Cell Proliferation Microscopy, Electron, Scanning Polylysine Rats, Sprague-dawley Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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