NDLI: Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Xueming Mooney, Paul Zheng, Shawn Booth, Christopher R. Braunfeld, Michael B. Gubbens, Sander Agard, David A. Cheng, Yifan
Description	Country affiliation: United States Author Affiliation: Li X ( The Keck Advanced Microscopy Laboratory, Department of Biochemistry and Biophysics, University of California, San Francisco (UCSF), San Francisco, California, USA.)
Abstract	In recent work with large high-symmetry viruses, single-particle electron cryomicroscopy (cryo-EM) has achieved the determination of near-atomic-resolution structures by allowing direct fitting of atomic models into experimental density maps. However, achieving this goal with smaller particles of lower symmetry remains challenging. Using a newly developed single electron-counting detector, we confirmed that electron beam-induced motion substantially degrades resolution, and we showed that the combination of rapid readout and nearly noiseless electron counting allow image blurring to be corrected to subpixel accuracy, restoring intrinsic image information to high resolution (Thon rings visible to â¼3 Å). Using this approach, we determined a 3.3-Å-resolution structure of an â¼700-kDa protein with D7 symmetry, the Thermoplasma acidophilum 20S proteasome, showing clear side-chain density. Our method greatly enhances image quality and data acquisition efficiency-key bottlenecks in applying near-atomic-resolution cryo-EM to a broad range of protein samples.
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.2472
Journal	Nature Methods
Issue Number	6
Volume Number	10
Language	English
Publisher	Nature Publishing Group
Publisher Date	2013-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Cryoelectron Microscopy Proteasome Endopeptidase Complex Ultrastructure Thermoplasma Enzymology Electrons Imaging, Three-dimensional Motion Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

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