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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Otto, Oliver Rosendahl, Philipp Mietke, Alexander Golfier, Stefan Herold, Christoph Klaue, Daniel Girardo, Salvatore Pagliara, Stefano Ekpenyong, Andrew Jacobi, Angela Wobus, Manja Töpfner, Nicole Keyser, Ulrich F. Mansfeld, Jörg Fischer-Friedrich, Elisabeth Guck, Jochen |
| Description | Country affiliation: Germany Author Affiliation: Otto O ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Rosendahl P ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Mietke A ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Golfier S ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Herold C ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Klaue D ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Girardo S ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Pagliara S ( Cavendish Laboratory, University of Cambridge, Cambridge, UK.); Ekpenyong A ( 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Department of Physics, Creighton University, Omaha, Nebraska, USA.); Jacobi A ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Wobus M ( Medizinische Klinik 1, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.); Töpfner N ( Klinik und Poliklinik für Kinder und Jugendmedizin, Universitätsklinikum Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.); Keyser UF ( Cavendish Laboratory, University of Cambridge, Cambridge, UK.); Mansfeld J ( Biotechnology Center, Technische Universität Dresden, Dresden, Germany.); Fischer-Friedrich E ( 1] Max Planck Institute for the Physics of Complex Systems, Dresden, Germany. [2] Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.); Guck J ( 1] Biotechnology Center, Technische Universität Dresden, Dresden, Germany. [2] Cavendish Laboratory, University of Cambridge, Cambridge, UK.) |
| Abstract | We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (>100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| Issue Number | 3 |
| Volume Number | 12 |
| e-ISSN | 15487105 |
| Journal | Nature Methods |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2015-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Flow Cytometry Instrumentation Methods Antigens, Cd34 Metabolism Cell Cycle Cell Differentiation Cell Lineage Cell Shape Cytochalasin D Pharmacology Cytoskeleton Equipment Design Hl-60 Cells Cytology Drug Effects Hematopoietic Stem Cells Humans Microfluidic Analytical Techniques Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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