NDLI: Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping.

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Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Van de Plas, Raf Yang, Junhai Spraggins, Jeffrey Caprioli, Richard M.
Description	Country affiliation: Netherlands Author Affiliation: Van de Plas R ( 1] Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA. [2] Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA. [3] Delft Center for Systems and Control, Delft University of Technology, Delft, the Netherlands.); Yang J ( 1] Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA. [2] Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA.); Spraggins J ( 1] Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA. [2] Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA.); Caprioli RM ( 1] Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA. [2] Department of Biochemistry, Vanderbilt University, Nashville, Tennessee, USA. [3] Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA. [4] Department of Pharmacology, Vanderbilt Universi)
Abstract	We describe a predictive imaging modality created by 'fusing' two distinct technologies: imaging mass spectrometry (IMS) and microscopy. IMS-generated molecular maps, rich in chemical information but having coarse spatial resolution, are combined with optical microscopy maps, which have relatively low chemical specificity but high spatial information. The resulting images combine the advantages of both technologies, enabling prediction of a molecular distribution both at high spatial resolution and with high chemical specificity. Multivariate regression is used to model variables in one technology, using variables from the other technology. We demonstrate the potential of image fusion through several applications: (i) 'sharpening' of IMS images, which uses microscopy measurements to predict ion distributions at a spatial resolution that exceeds that of measured ion images by ten times or more; (ii) prediction of ion distributions in tissue areas that were not measured by IMS; and (iii) enrichment of biological signals and attenuation of instrumental artifacts, revealing insights not easily extracted from either microscopy or IMS individually.
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.3296
Journal	Nature Methods
Issue Number	4
Volume Number	12
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Image Processing, Computer-assisted Mass Spectrometry Microscopy Animals Brain Ultrastructure Instrumentation Mice Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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