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Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pasca, Anca M. Sloan, Steven A. Clarke, Laura E. Tian, Yuan Makinson, Christopher D. Huber, Nina Kim, Chul Hoon Park, Jin-Young O'Rourke, Nancy A. Nguyen, Khoa D. Smith, Stephen J. Huguenard, John R. Geschwind, Daniel H. Barres, Ben A. Pasca, Sergiu P.
Description	Country affiliation: United States Author Affiliation: Pasca AM ( Department of Pediatrics, Division of Neonatology, Stanford University School of Medicine, Stanford, California, USA.); Sloan SA ( Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.); Clarke LE ( Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.); Tian Y ( 1] Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, California, USA. [2] Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, California, USA. [3] Interdepartmental Ph.D. Program in Bioinformatics, Unive); Makinson CD ( Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California, USA.); Huber N ( Department of Psychiatry &Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, California, USA.); Kim CH ( 1] Department of Pharmacology, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea. [2] BK21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.); Park JY ( Department of Psychiatry &Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, California, USA.); O'Rourke NA ( Department of Molecular and Cellular Physiology, Beckman Center, Stanford University School of Medicine, Stanford, California, USA.); Nguyen KD ( Department of Pathology, Blood Center, Stanford University School of Medicine, Stanford, California, USA.); Smith SJ ( 1] Department of Molecular and Cellular Physiology, Beckman Center, Stanford University School of Medicine, Stanford, California, USA. [2] Department of Synapse Biology, Allen Institute for Brain Science, Seattle, Washington, USA.); Huguenard JR ( Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, California, USA.); Geschwind DH ( 1] Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, California, USA. [2] Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, California, USA. [3] Interdepartmental Ph.D. Program in Bioinformatics, Unive); Barres BA ( Department of Neurobiology, Stanford University School of Medicine, Stanford, California, USA.); Pasca SP ( Department of Psychiatry &Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, California, USA.)
Abstract	The human cerebral cortex develops through an elaborate succession of cellular events that, when disrupted, can lead to neuropsychiatric disease. The ability to reprogram somatic cells into pluripotent cells that can be differentiated in vitro provides a unique opportunity to study normal and abnormal corticogenesis. Here, we present a simple and reproducible 3D culture approach for generating a laminated cerebral cortex-like structure, named human cortical spheroids (hCSs), from pluripotent stem cells. hCSs contain neurons from both deep and superficial cortical layers and map transcriptionally to in vivo fetal development. These neurons are electrophysiologically mature, display spontaneous activity, are surrounded by nonreactive astrocytes and form functional synapses. Experiments in acute hCS slices demonstrate that cortical neurons participate in network activity and produce complex synaptic events. These 3D cultures should allow a detailed interrogation of human cortical development, function and disease, and may prove a versatile platform for generating other neuronal and glial subtypes in vitro.
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.3415
Journal	Nature Methods
Issue Number	7
Volume Number	12
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Astrocytes Physiology Cerebral Cortex Pluripotent Stem Cells Cytology Cells, Cultured Spheroids, Cellular Synapses Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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