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Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells.

Year: 2008 Volume: 7 Issue: 5

Year: 2008 Volume: 7 Issue: 4

Year: 2008 Volume: 7 Issue: 2

Year: 2008 Volume: 7 Issue: 1

Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tan, Hwee Tong Tan, Sandra Lin, Qingsong Lim, Teck Kwang Hew, Choy Leong Chung, Maxey C. M.
Description	Country affiliation: Singapore Author Affiliation: Tan HT ( Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 117597, Singapore.)
Abstract	Colorectal cancer is one of the most common cancers in developed countries, and its incidence is negatively associated with high dietary fiber intake. Butyrate, a short-chain fatty acid fermentation by-product of fiber induces cell maturation with the promotion of growth arrest, differentiation, and/or apoptosis of cancer cells. The stimulation of cell maturation by butyrate in colonic cancer cells follows a temporal progression from the early phase of growth arrest to the activation of apoptotic cascades. Previously we performed two-dimensional DIGE to identify differentially expressed proteins induced by 24-h butyrate treatment of HCT-116 colorectal cancer cells. Herein we used quantitative proteomics approaches using iTRAQ (isobaric tags for relative and absolute quantitation), a stable isotope labeling methodology that enables multiplexing of four samples, for a temporal study of HCT-116 cells treated with butyrate. In addition, cleavable ICAT, which selectively tags cysteine-containing proteins, was also used, and the results complemented those obtained from the iTRAQ strategy. Selected protein targets were validated by real time PCR and Western blotting. A model is proposed to illustrate our findings from this temporal analysis of the butyrate-responsive proteome that uncovered several integrated cellular processes and pathways involved in growth arrest, apoptosis, and metastasis. These signature clusters of butyrate-regulated pathways are potential targets for novel chemopreventive and therapeutic drugs for treatment of colorectal cancer.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
Journal	Molecular & Cellular Proteomics
Issue Number	6
Volume Number	7
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2008-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Butyrates Pharmacology Colorectal Neoplasms Drug Therapy Gene Expression Regulation, Neoplastic Proteomics Apoptosis Cell Cycle Cell Line, Tumor Cysteine Chemistry False Positive Reactions Mass Spectrometry Models, Biological Peptides Reverse Transcriptase Polymerase Chain Reaction Time Factors Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

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