NDLI: Characterization of membrane-shed microvesicles from cytokine-stimulated ß-cells using proteomics strategies.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Palmisano, Giuseppe Jensen, Søren Skov Le Bihan, Marie-Catherine Lainé, Jeanne McGuire, James N. Pociot, Flemming Larsen, Martin Røssel
Description	Country affiliation: Denmark Author Affiliation: Palmisano G ( Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.)
Abstract	Microparticles and exosomes are two of the most well characterized membrane-derived microvesicles released either directly from the plasma membrane or released through the fusion of intracellular multivesicular bodies with the plasma membrane, respectively. They are thought to be involved in many significant biological processes such as cell to cell communication, rescue from apoptosis, and immunological responses. Here we report for the first time a quantitative study of proteins from ß-cell-derived microvesicles generated after cytokine induced apoptosis using stable isotope labeled amino acids in cell culture combined with mass spectrometry. We identified and quantified a large number of ß-cell-specific proteins and proteins previously described in microvesicles from other cell types in addition to new proteins located to these vesicles. In addition, we quantified specific sites of protein phosphorylation and N-linked sialylation in proteins associated with microvesicles from ß-cells. Using pathway analysis software, we were able to map the most distinctive changes between microvesicles generated during growth and after cytokine stimulation to several cell death and cell signaling molecules including tumor necrosis factor receptor superfamily member 1A, tumor necrosis factor, -induced protein 3, tumor necrosis factor-interacting kinase receptor-interacting serine-threonine kinase 1, and intercellular adhesion molecule 1.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
DOI	10.1074/mcp.M111.012732
Journal	Molecular & Cellular Proteomics
Issue Number	8
Volume Number	11
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2012-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Cell-derived Microparticles Metabolism Cytokines Pharmacology Exosomes Insulin-secreting Cells Drug Effects Proteomics Amino Acids Animals Apoptosis Carbon Isotopes Cell Line, Tumor Cell Membrane Ultrastructure Chromatography, Liquid Glycoproteins Pathology Interferon-gamma Interleukin-1beta Isotope Labeling Microscopy, Electron, Transmission Microscopy, Fluorescence Nitrogen Isotopes Phosphoproteins Tandem Mass Spectrometry Tumor Necrosis Factor-alpha Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Myricetin protects against cytokine-induced cell death in RIN-m5f ß cells.

Characterization of Membrane-shed Microvesicles from Cytokine-stimulated β-Cells Using Proteomics Strategies*

Tumor necrosis factor and immune interferon act in concert to slow the lateral diffusion of proteins and lipids in human endothelial cell membranes

Stable isotope labeling of phosphoproteins for large-scale phosphorylation rate determination.

Circulating microvesicles and exosomes in small cell lung cancer by quantitative proteomics

Circulating microparticles: square the circle

Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.

Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic ß- and -Cells.

Quantification of the N-glycosylated secretome by super-SILAC during breast cancer progression and in human blood samples.

Characterization of membrane-shed microvesicles from cytokine-stimulated ß-cells using proteomics strategies.

Similar Documents

Myricetin protects against cytokine-induced cell death in RIN-m5f ß cells.

Characterization of Membrane-shed Microvesicles from Cytokine-stimulated β-Cells Using Proteomics Strategies*

Tumor necrosis factor and immune interferon act in concert to slow the lateral diffusion of proteins and lipids in human endothelial cell membranes

Stable isotope labeling of phosphoproteins for large-scale phosphorylation rate determination.

Circulating microvesicles and exosomes in small cell lung cancer by quantitative proteomics

Circulating microparticles: square the circle

Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids.

Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic ß- and -Cells.

Quantification of the N-glycosylated secretome by super-SILAC during breast cancer progression and in human blood samples.

Characterization of membrane-shed microvesicles from cytokine-stimulated ß-cells using proteomics strategies.