NDLI: Wnt3a-dependent and -independent protein interaction networks of chromatin-bound ß-catenin in mouse embryonic stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yakulov, Toma Raggioli, Angelo Franz, Henriette Kemler, Rolf
Description	Country affiliation: Germany Author Affiliation: Yakulov T ( Department of Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.)
Abstract	Canonical Wnt signaling is repeatedly used during development to control cell fate, and it is often implicated in human cancer. ß-catenin, the effector of Wnt signaling, has a dual function in the cell and is involved in both cell adhesion and transcription. Nuclear ß-catenin controls transcription through association with transcription factors of the TCF family and the recruitment of epigenetic modifiers. In this study, we used a strategy combining the genetic manipulation of mouse embryonic stem cells with affinity purification and quantitative mass spectroscopy utilizing stable isotope labeling with amino acids in cell culture to study the interactome of chromatin-bound ß-catenin with and without Wnt3a stimulation. We uncovered previously unknown interactions of ß-catenin with transcription factors and chromatin-modifying complexes. Our proof-of-principle experiments show that ß-catenin can recruit the H3K4me2/1 demethylase LSD1 to regulate the expression of the tumor suppressor Lefty1 in mouse embryonic stem cells. The mRNA levels of LSD1 and ß-catenin are inversely correlated with the levels of Lefty1 in pancreas and breast tumors, implying that this mechanism is common to mouse embryonic stem cells and cancer cells.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
DOI	10.1074/mcp.M112.026914
Journal	Molecular & Cellular Proteomics
Issue Number	7
Volume Number	12
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2013-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Chromatin Metabolism Embryonic Stem Cells Left-right Determination Factors Oxidoreductases, N-demethylating Wnt3a Protein Pharmacology Beta Catenin Animals Breast Neoplasms Cell Line Histone Demethylases Genetics Mice Neoplasm Proteins Pancreatic Neoplasms Protein Interaction Maps Rna, Messenger Tamoxifen
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

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