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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Narayan, Kartik Lemmon, Mark A. |
| Description | Country affiliation: United States Author Affiliation: Narayan K ( Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA.) |
| Abstract | The burgeoning of phosphoinositide-binding domains and proteins in cellular signaling and trafficking has drawn laboratories from a wide variety of fields into the study of lipid interactions with peripheral membrane proteins. Many different approaches have been developed to assess phosphoinositide binding, some of which are more problematic than others, and some of which can be quantitated more readily than others. With a focus on the methods used in our laboratory, we describe here the considerations that need to be taken into account when establishing-and quantitating-the specific binding of a protein or domain to phosphoinositides in membranes. We also discuss briefly a few examples in which no clear consensus has yet been reached as to the specificity of a given domain or protein because of discrepancies between different commonly used approaches. |
| File Format | HTM / HTML |
| ISSN | 10462023 |
| e-ISSN | 10959130 |
| Journal | Methods |
| Issue Number | 2 |
| Volume Number | 39 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2006-06-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Life sciences, Biochemistry Biochemistry Lipids Phosphatidylinositols Metabolism Protein Structure, Tertiary Proteins Binding Sites Blotting, Western Calorimetry Kinetics Phospholipids Phosphorus Radioisotopes Substrate Specificity Surface Plasmon Resonance Research Support, N.i.h., Extramural |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology |
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