NDLI: Using molecular tethering to analyze the role of nuclear compartmentalization in the regulation of mammalian gene activity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Reddy, K. L. Singh, H.
Description	Country affiliation: United States Author Affiliation: Reddy KL ( Department of Molecular Genetics and Cell Biology, The University of Chicago, Howard Hughes Medical Institute, GCIS W522, 929 E 57th Street, Chicago, IL 60637, USA.)
Abstract	The mammalian nucleus has a complex structural organization that dynamically interacts with the genome. Chromatin is organized into discrete domains by association with distinct nuclear compartments enriched in structural and regulatory proteins. Growing evidence suggests that gene activity is modulated by interactions with these sub-nuclear compartments. Therefore, analyzing how nuclear architecture controls genome activity will be necessary to fully understand complex biological processes such as development and disease. In this article we describe a molecular methodology involving inducible tethering that can be used to position genes at the inner nuclear membrane (INM)-lamina compartment. The consequences of such directed re-positioning on gene activity or other DNA transactions can then be analyzed. This approach can be generalized and extended to position genes or chromosomal domains within other nuclear compartments thereby greatly facilitating the analysis of nuclear structure and its impact on genome activity.
File Format	HTM / HTML
ISSN	10462023
e-ISSN	10959130
DOI	10.1016/j.ymeth.2008.06.013
Journal	Methods
Issue Number	3
Volume Number	45
Language	English
Publisher	Elsevier
Publisher Date	2008-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Life sciences, Biochemistry Chromosome Positioning Dna-binding Proteins In Situ Hybridization, Fluorescence Microscopy, Fluorescence Recombinant Fusion Proteins Animals Bacterial Proteins Genetics Cell Line Chromatin Metabolism Chromatin Assembly And Disassembly Physiology Electroporation Genes, Reporter Green Fluorescent Proteins Lac Operon Luminescent Proteins Mammals Membrane Proteins Nuclear Matrix Nuclear Proteins Nucleic Acid Probes Thymidine Kinase Transcription, Genetic Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology

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