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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nagai, Hiroki Sezaki, Maiko Nakamura, Harukazu Sheng, Guojun |
| Description | Country affiliation: Japan Author Affiliation: Nagai H ( Lab for Early Embryogenesis, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-Ku, Kobe, Hyogo 650 0047, Japan.); Sezaki M ( Lab for Early Embryogenesis, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-Ku, Kobe, Hyogo 650 0047, Japan.); Nakamura H ( Department of Molecular Neurobiology, Graduate School of Life Sciences, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai, Japan.); Sheng G ( Lab for Early Embryogenesis, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-Ku, Kobe, Hyogo 650 0047, Japan. Electronic address: sheng@cdb.riken.jp.) |
| Abstract | The New and Early Chick (EC) methods, two commonly used techniques for ex ovo culture of early-stage avian embryos, are limited by poor survivability after initiation of circulation. This limitation is circumvented with two recent technical advancements: the modified Cornish pasty culture and whole-embryo transplantation. The former supports optimal ex ovo growth till stage HH18, and the latter allows ex-ovo-manipulated embryos to have long-term in ovo-survivability. Here we provide step-by-step instructions for both methods. These two new techniques can also be combined to achieve targeted labeling, imaging and electroporation in early-stage embryos ex ovo, and phenotypic and functional analyses at more advanced developmental stages in ovo. |
| File Format | HTM / HTML |
| ISSN | 10462023 |
| Issue Number | 3 |
| Journal | Methods |
| Volume Number | 66 |
| e-ISSN | 10959130 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-04-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Life sciences, Biochemistry Birds Embryology Embryo, Nonmammalian Tissue Culture Techniques Animals Chick Embryo Embryonic Development Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology |
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