NDLI: False-negative beta-D-glucuronidase reactions in membrane lactose glucuronide agar medium used for the simultaneous detection of coliforms and Escherichia coli from water.

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False-negative beta-D-glucuronidase reactions in membrane lactose glucuronide agar medium used for the simultaneous detection of coliforms and Escherichia coli from water.

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False-negative beta-D-glucuronidase reactions in membrane lactose glucuronide agar medium used for the simultaneous detection of coliforms and Escherichia coli from water.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fricker, C. R. DeSarno, M. Warden, P. S. Eldred, B. J.
Description	Country affiliation: United States Author Affiliation: Fricker CR ( Analytical Services Inc., Allen Brook Lane, Williston, Vermont, USA. colinfricker@aol.com)
Abstract	AIMS: Testing for beta-D-glucuronidase activity has become the basis of many methods for the detection of Escherichia coli in both food and water. Used in combination with tests for the presence of beta-D-glucuronidase, these tests offer a simple method for simultaneously detecting coliforms and E. coli. The purpose of this study was to determine the effectiveness of several different procedures in detecting beta-D-glucuronidase activity and hence in detecting E. coli. METHODS AND RESULTS: The ability of membrane lactose glucuronide agar (MLGA), Colilert-18, MI agar, Colitag and Chromocult agar to detect beta-D-glucuronidase activity was tested with over 1000 isolates of E. coli recovered from naturally contaminated water samples. Four of the media gave very similar results but MLGA failed to detect glucuronidase activity in 15.6% of the cultures tested. CONCLUSIONS: MLGA had very poor sensitivity for the detection of beta-D-glucuronidase activity in strains of E. coli isolated from naturally contaminated water. This is probably because of the fact that beta-D-glucuronidase activity is pH-sensitive and that acid is formed by E. coli during fermentation of lactose in MLGA. SIGNIFICANCE AND IMPACT OF THE STUDY: The detection of E. coli in drinking water is the primary test used to establish faecal contamination. The poor sensitivity of MLGA in detecting beta-D-glucuronidase activity suggests that this medium and others containing high concentrations of fermentable carbohydrate should not be used for the detection of E. coli.
File Format	HTM / HTML
ISSN	02668254
Issue Number	6
Volume Number	47
e-ISSN	1472765X
Journal	Letters in Applied Microbiology
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2008-12-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Biochemistry Standards Culture Media Chemistry Escherichia Coli Proteins Analysis Escherichia Coli Isolation & Purification Glucuronidase Water Microbiology Water Pollutants Methods Metabolism Enzymology Glucuronides Lactose Sensitivity And Specificity Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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False-negative beta-D-glucuronidase reactions in membrane lactose glucuronide agar medium used for the simultaneous detection of coliforms and Escherichia coli from water.

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