NDLI: Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum.

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Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Okibe, N. Suzuki, N. Inui, M. Yukawa, H.
Description	Country affiliation: Japan Author Affiliation: Okibe N ( Research Institute of Innovative Technology for the Earth, Kizugawadai, Kizugawa, Kyoto, Japan.)
Abstract	AIMS: To obtain strong, carbon source-inducible promoters useful for industrial applications of Corynebacterium glutamicum. METHODS AND RESULTS: DNA microarray and qRT-PCR enabled identification of the promoters of cgR_2367 (malE1) and cgR_2459 (git1) as strong, maltose- and gluconate-inducible promoters, respectively, in C. glutamicum. Promoter probe assays revealed that in the presence of the inducing sugars, PmalE1 and Pgit1, respectively, facilitated 3.4- and 4.2-fold increased beta-galactosidase activities compared to the same activity induced by glucose. In addition, PmalE1 was not functional in Escherichia coli, in which Pgit1 function was repressible, which enabled the cloning of a hitherto 'difficult-to-clone' heterologous gene of a lignocellulolytic enzyme, whose secretion was consequently induced by the carbon sources. CONCLUSIONS: PmalE1 and Pgit1 are strong, carbon source-inducible promoters of C. glutamicum whose characteristics in E. coli are integral to the secretion ability of C. glutamicum to secrete lignocellulolytic enzyme. SIGNIFICANCE AND IMPACT OF THE STUDY: Corynebacterium glutamicum, like its counterpart industrial workhorses E. coli and Bacillus subtilis, does exhibit strong, carbon source-inducible promoters, and the functionality of two of which was demonstrated in this study. While this study may be most relevant in the ongoing efforts to establish technologies of the biorefinery, it should also be of interest to general microbiologists exploring the versatility of industrial micro-organisms. In so doing, the study should impact future advances in industrial microbiology.
File Format	HTM / HTML
ISSN	02668254
Issue Number	2
Volume Number	50
e-ISSN	1472765X
Journal	Letters in Applied Microbiology
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2010-02-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Carbon Corynebacterium Glutamicum Genetics Gene Expression Regulation, Bacterial Physiology Industrial Microbiology Promoter Regions, Genetic Bacillus Subtilis Carbohydrates Pharmacology Escherichia Coli Drug Effects Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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