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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Uchio, Eiichi Inoue, Hirotoshi Kadonosono, Kazuaki |
| Description | Responsible library: WPRO |
| Abstract | PURPOSE: Antimicrobial peptides have an important role in self-protection of the ocular surface. Human cationic antimicrobial protein (hCAP)-18 is a linear, alpha-helical peptide that consists of a conserved pro-sequence called a cathelin-like domain and a C-terminal peptide named LL-37. We investigated the in vitro anti-adenoviral activity of hCAP-18/LL-37 in several adenovirus types, inducing keratoconjunctivitis. METHODS: A549 cells were used for viral cell culture, and human adenovirus (HAdV) types 3 (HAdV3, species B), 4 (species E), 8, 19a, and 37 (species D) were used. The cytotoxicity of LL-37 was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay to obtain 50% cytotoxic concentration. After pretreatment of A549 cells with serial dilutions of LL-37 for 24 hours, adenovirus was cultured for seven days, and adenoviral DNA was quantitatively measured by real-time polymerase chain reaction (PCR). RESULTS: The 50% effective concentration of LL-37 obtained by real-time PCR ranged between 118 and 270 microM. LL-37 showed a significant inhibitory effect on adenoviral proliferation in all adenovirus types except HAdV4 in a dose-dependent manner. CONCLUSIONS: LL-37 has significant inhibitory activity against HAdV3, 8, and 19, which induce keratoconjunctivitis. These results indicate that hCAP-18/LL-37 may be a possible candidate for the treatment of HAdV keratoconjunctivitis. |
| File Format | HTM / HTML |
| ISSN | 10118942 |
| e-ISSN | 20929382 |
| Journal | Korean Journal of Ophthalmology |
| Issue Number | 3 |
| Volume Number | 27 |
| Language | English |
| Publisher | Korean Ophthalmological Society |
| Publisher Date | 2013-01-01 |
| Publisher Place | Korea (South) |
| Access Restriction | Open |
| Subject Keyword | Discipline Ophthalmology Adenoviridae Adenoviruses, Human Antiviral Agents Cell Culture Techniques Conjunctivitis, Viral Keratoconjunctivitis Peptides Polymerase Chain Reaction Real-time Polymerase Chain Reaction In Vitro Original Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ophthalmology |
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