NDLI: Antigenic properties of cystatin-binding cysteine proteinases from Neodiplostomum seoulense.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Tae-Yun Han, Kye-Young Shin, Eun-Hee Chai, Jong-Yil
Description	Author Affiliation: Kim TY ( Department of Infection Biology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea.)
Abstract	The antigenic properties of cysteine proteinases binding to cystatin were analyzed in Neodiplostomum seoulense, an intestinal trematode that infects humans and rodents in the Republic of Korea. Cystatin was found to effectively capture cysteine proteinases present in the crude extract of N. seoulense. The IgG levels against cystatin-binding cysteine proteinases in sera of mice infected with N. seoulense were higher than those in sera of mice immunized with the crude extract of N. seoulense. The production of IgG antibodies against cystatin-binding cysteine proteinases increased according to the length of infection period. In immunoblots of purified cystatin-binding proteinases, 2 molecules, approximately 50 kDa and 60 kDa, reacted with N. seoulense-infected mouse sera. Of the sera from patients infected with various helminths, those of sparganum-infected patients showed the strongest affinities for cystatin-binding cysteine proteinases of N. seoulense. Cystatin-binding cysteine proteinases of N. seoulense are suggested to be putative antigens for serodiagnosis of human N. seoulense infection.
File Format	HTM / HTML
ISSN	00223395
Issue Number	3
Volume Number	94
e-ISSN	19372345
Journal	Journal of Parasitology
Language	English
Publisher	American Society of Parasitologists
Publisher Date	2008-06-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Parasitology Antigens, Helminth Immunology Cystatins Metabolism Cysteine Endopeptidases Trematoda Enzymology Animals Antibodies, Helminth Biosynthesis Carrier Proteins Chemistry Enzyme-linked Immunosorbent Assay Immune Sera Immunoblotting Immunoglobulin G Mice Mice, Inbred Balb C Mice, Inbred C3h Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Parasitology Ecology, Evolution, Behavior and Systematics

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