NDLI: Khz (fusion product of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in human colon carcinoma HCT116 cells, accompanied by an increase in reactive oxygen species, activation of caspase 3, and increased intracellular Ca²âº.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Tae Hwan Kim, Ju Sung Kim, Zoo Haye Huang, Ren Bin Chae, Young Lye Wang, Ren Sheng
Description	Country affiliation: China Author Affiliation: Kim TH ( 1 Department of Radiotherapy, The First Affiliated Hospital, Guangxi Medical University , Nanning, China .)
Abstract	Khz (a fusion mycelium of Ganoderma lucidum and Polyporus umbellatus mycelia) is isolated from ganoderic acid and P. umbellatus and it exerts antiproliferative effects against malignant cells. However, no previous study has reported the inhibitory effects of Khz on the growth of human colon cancer cells. In the present study, we found that Khz suppressed cell division and induced apoptosis in HCT116 cells. Khz cytotoxicity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Khz reduced cell viability and mitochondrial membrane potential levels and it also induced disruption of the mitochondrial membrane potential and increased calcium concentration and reactive oxygen species generation. Khz increased caspase 3, PARP, caspase 7, and caspase 9 levels, but reduced Bcl-2 protein levels. Flow cytometry showed that the percentage of HCT116 cells in the sub-G1 phase of the cell cycle increased in response to Khz treatment.
File Format	HTM / HTML
ISSN	1096620X
Issue Number	3
Volume Number	18
e-ISSN	15577600
Journal	Journal of Medicinal Food
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2015-03-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Nutritional Sciences Antineoplastic Agents Pharmacology Apoptosis Drug Effects Biological Products Colon Colorectal Neoplasms Drug Therapy Polyporus Reishi Therapeutic Use Calcium Metabolism Caspase 3 Cell Cycle Cytology Enzyme Activation G1 Phase Hct116 Cells Humans Membrane Potential, Mitochondrial Mycelium Reactive Oxygen Species Journal Article
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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Khz (fusion product of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in human colon carcinoma HCT116 cells, accompanied by an increase in reactive oxygen species, activation of caspase 3, and increased intracellular Ca²âº.

Khz (fusion product of Ganoderma lucidum and Polyporus umbellatus mycelia) induces apoptosis in human colon carcinoma HCT116 cells, accompanied by an increase in reactive oxygen species, activation of caspase 3, and increased intracellular Ca²âº.