NDLI: Anti-Inflammatory Activity of Butein and Luteolin Through Suppression of NFκB Activation and Induction of Heme Oxygenase-1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sung, Jeehye Lee, Junsoo
Description	Author Affiliation: Sung J ( Department of Food Science and Biotechnology, College of Agriculture, Life & Environmental Sciences, Chungbuk National University , Cheongju, Chungbuk, Korea.)
Abstract	Butein and luteolin are members of the flavonoid family, which displays a variety of biological activities. In this study, we demonstrated that butein and luteolin exert anti-inflammatory activities in RAW264.7 macrophages by inducing heme oxygenase-1 (HO-1) expression. Butein and luteolin dose-dependently attenuated inducible nitric oxide synthase (iNOS) expression, leading to the suppression of iNOS-derived nitric oxide (NO) production. The inhibitory effect of butein on NO production was greater than that of luteolin. Consistent with this finding, butein also showed higher inhibitory effects on lipopolysaccharide (LPS)-induced translocation of nuclear factor κB (NFκB) and NFκB reporter gene activity in macrophages than luteolin. Furthermore, the expression of HO-1 was dose-dependently induced by butein and luteolin treatments in macrophages. Additionally, the anti-inflammatory activities of butein and luteolin involved the induction of HO-1 expression, as confirmed by the zinc protoporphyrin (ZnPP) treatment (HO-1 selective inhibitor) and HO-1 small interfering (si)RNA system. ZnPP-mediated downregulation and siRNA-mediated knockdown of HO-1 significantly abolished the inhibitory effects of butein and luteolin on the production of NO in LPS-induced macrophages. Consequently, butein and luteolin were shown to be effective HO-1 inducers capable of inhibiting macrophage-derived proinflammatory mechanisms. These findings indicate that butein and luteolin are potential therapeutic agents for the treatment of inflammatory diseases.
File Format	HTM / HTML
ISSN	1096620X
Issue Number	5
Volume Number	18
e-ISSN	15577600
Journal	Journal of Medicinal Food
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2015-05-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Nutritional Sciences Anti-inflammatory Agents Pharmacology Chalcones Heme Oxygenase-1 Genetics Luteolin Nf-kappa B Animals Immunology Macrophages Drug Effects Enzymology Mice Nitric Oxide Synthase Type Ii Raw 264.7 Cells Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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