NDLI: Pattern of immunoglobulin and T-cell receptor-Î´/Î³ gene rearrangements in Iranian children with B-precursor acute lymphoblastic leukemia.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Poopak, Behzad Saki, Najmaldin Purfatholah, Ali Akbar Najmabadi, Hossein Mortazavi, Yosef Arzanian, Mohammad T. Khosravipour, Gelareh Haghnejad, Fariba Salari, Fatemeh Shahjahani, Mohammad
Spatial Coverage	Iran
Abstract	INTRODUCTION: Acute lymphoblastic leukemia (ALL) cells have unique rearranged immunoglobulin heavy chain (IgH), immunoglobulin light chain (IgK), and T-cell receptor (TCR) genes, which can be used as markers for clonality assay and evaluation of minimal residual disease. In this study, we have evaluated the pattern of IgH, IgK chains, and TCRG/D gene rearrangements in precursor-B ALL. MATERIALS AND METHODS: In our prospective study, hyper-variable regions (CDRI and III) of IgH, TCRD (VÎ´2-DÎ´3 and DÎ´2-DÎ´3), TCRG (VÎ³, VÎ³I, and VÎ³II), and IgK (Vκ-Kde) were studied in 126 cases with diagnosis of B-precursor ALL. RESULTS: One hundred and fourteen (90.5%) out of 126 patients had clonal rearrangements of IgH using consensus primers for CDRI and/or CDRIII regions. Monoclonal, biclonal, and oligoclonal patterns were observed in 63 (57.8%), 38 (34.9%), and 6 (5.5%) patients with IgH (CDRIII) rearrangements, respectively. Clonal rearrangements of TCRG (VÎ³) and VÎ³I/II were present in 79.3 and 64.9% of patients, respectively, and only 5% of cases showed biclonal pattern. The VÎ³II rearrangement was the most common (46.8%) type in TCRG. VÎ´2-DÎ´3 and DÎ´2-DÎ´3 partial gene rearrangements were observed in 47 (45.2%; n = 104) and 11 (16.6%; n = 66) patients, respectively. Biclonal/oligoclonal patterns were present in 13 (27.7%) and 2 (4.3%) cases with VÎ´2-DÎ´3 rearrangement, respectively. Only one patient had biclonal DÎ´2-DÎ´3 rearrangement. Clonal pattern of IgK-Kde was detected in 59 cases (67%; n = 88). CONCLUSION: Our findings showed that clonal rearrangements of IgH and TCRD (VÎ´2-DÎ´3 and DÎ´2-DÎ´3) genes had similar patterns to other studies. Frequency of TCRG (VÎ³I and VÎ³II) and IgK rearrangements was found to be slightly higher than previous reports. Among the IgK rearrangements, VKI (25%) was the most common.
File Format	HTM / HTML
ISSN	10245332
Issue Number	5
Volume Number	19
e-ISSN	16078454
Journal	Hematology
Language	English
Publisher	Taylor & Francis
Publisher Date	2014-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Hematology Gene Rearrangement, B-lymphocyte Gene Rearrangement, T-lymphocyte Immunoglobulins Genetics Precursor B-cell Lymphoblastic Leukemia-lymphoma Receptors, Antigen, T-cell, Gamma-delta Bone Marrow Pathology Child Child, Preschool Female Humans Immunoglobulin Kappa-chains Immunophenotyping Infant Iran Male Diagnosis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Hematology

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