NDLI: Prevalence and characterization of Escherichia coli O157:H7 isolates from meat and meat products sold in Amathole District, Eastern Cape Province of South Africa.

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Year: 2009 Volume: 26 Issue: 1

Year: 2008 Volume: 25

Prevalence and characterization of Escherichia coli O157:H7 isolates from meat and meat products sold in Amathole District, Eastern Cape Province of South Africa.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Abong'o, Benard O. Momba, Maggy N. B.
Spatial Coverage	South Africa
Description	Country affiliation: South Africa Author Affiliation: Abong'o BO ( University of Fort Hare, Department of Biochemistry and Microbiology, School of Science and Technology, P/Bag X1314, Alice 5700, South Africa. melfelis@gmail.com)
Abstract	Meat and meat products have been implicated in outbreaks of Escherichia coli O157:H7 in most parts of the world. In the Amathole District Municipality of the Eastern Cape Province of South Africa, a large number of households consume meat and meat products daily, although the microbiological quality of these types of food is questionable. The present study investigated the prevalence of E. coli O157:H7 isolated from selected meat and meat products (45 samples each of biltong, cold meat, mincemeat, and polony) sold in this area. Strains of E. coli O157:H7 were isolated by enrichment culture and confirmed by polymerase chain reaction (PCR). Also investigated were the antibiogram profiles of the E. coli O157:H7 isolates. Five (2.8%) out of 180 meat and meat products examined were positive for E. coli O157:H7 that carried the fliC(H7), rfbE(O157), and eaeA genes. Two of the E. coli O157:H7 isolates were resistant against all the eight antibiotics tested. To prevent E. coli O157:H7 infections, meat and meat products such as biltong, cold meat, mincemeat and polony should be properly handled, and packed in sterile polyvinyl wrappers.
File Format	HTM / HTML
ISSN	07400020
Issue Number	2
Volume Number	26
e-ISSN	10959998
Journal	Food Microbiology
Language	English
Publisher	Elsevier
Publisher Date	2009-04-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Nutritional Sciences Anti-bacterial Agents Pharmacology Drug Resistance, Bacterial Escherichia Coli O157 Isolation & Purification Food Contamination Analysis Food Handling Methods Meat Microbiology Colony Count, Microbial Consumer Product Safety Dna, Bacterial Chemistry Genetics Drug Effects Standards Food Microbiology Gene Amplification Humans Hygiene Meat Products Microbial Sensitivity Tests Prevalence South Africa Epidemiology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Microbiology Food Science

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