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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zamaratskaia, Galia Zlabek, Vladimir |
| Description | Country affiliation: Sweden Author Affiliation: Zamaratskaia G ( Department of Food Science, BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden. Galia.Zamaratskaia@lmv.slu.se) |
| Abstract | The study investigated the kinetics of p-nitrophenol hydroxylase (PNPH) in hepatic microsomes obtained from Atlantic salmon (Salmo salar). The selective inhibitors for some major mammalian cytochrome P450 (CYP450) were used to investigate the potential inhibitory effect on enzymes involved in p-nitrophenol hydroxylation. The following inhibitors were used: -naphtoflavone (CYP1A), ellipticine (CYP1A1), furafylline (CYP1A2), 8-methoxypsoralen (8MOP, CYP2A6), 4-methylpyrazole (4MP, CYP2A6/2E1), diallyl sulfide (DAS, CYP2E1), and ketoconazole (CYP3A4). Additionally, the natural steroids 17-beta-oestraiol (E2) and testosterone were investigated as potential inhibitors of PNPH activity. It was found that formation of 4-nitrocatechol from p-nitrophenol followed monophasic kinetics with K(m) = 0.17 ± 0.03 mM and V(max) = 21.8 ± 1.05 pmol/min/mg. PNPH activity was competitively inhibited by diallyle sulfide with the K(i) value of 285.1 ± 94.2 µM µM and uncompetitively by ellipticine with K(i) value of 65.7 ± 7.8 µM. Moreover, E2 showed an ability to reduce PNPH activity through the mechanism-based inhibition mode. Our results suggest that hepatic microsomes from Atlantic salmon possess CYP2E1-like activity. However, specific isoform-mediated PNPH activity should be identified. |
| File Format | HTM / HTML |
| ISSN | 09201742 |
| Issue Number | 4 |
| Volume Number | 37 |
| e-ISSN | 15735168 |
| Journal | Fish Physiology and Biochemistry |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2011-12-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Physiology Discipline Biochemistry Discipline Zoology Cytochrome P-450 Cyp2e1 Metabolism Microsomes, Liver Enzymology Nitrophenols Salmo Salar Physiology Animals Biotransformation Cytochrome P-450 Cyp2e1 Inhibitors Hydroxylation Kinetics Xenobiotics Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Biochemistry Aquatic Science |
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