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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Marotti, Jonathan D. Schwab, Mary C. McNulty, Nancy J. Rigas, James R. DeLong, Peter A. Memoli, Vincent A. Tsongalis, Gregory J. Padmanabhan, Vijayalakshmi |
| Description | Country affiliation: United States Author Affiliation: Marotti JD ( Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA. jonathan.d.marotti@hitchcock.org) |
| Abstract | Associations between bronchioloalveolar carcinoma (BAC), mucinous differentiation, and epidermal growth factor receptor (EGFR) and KRAS mutations have been previously reported in studies of surgical specimens. We present the cytomorphology of lung adenocarcinomas, including metastases that were diagnosed by cytologic methods and the relationship to both EGFR and KRAS mutational status. We retrospectively reviewed the clinical and cytomorphologic features of 50 lung adenocarcinomas that were tested for both EGFR and KRAS mutations. Cytomorphologic features evaluated included cell size, architectural pattern, nucleoli, intranuclear cytoplasmic inclusions (INCI), mucin, necrosis, squamoid features, lymphocytic response, and histologic features of BAC differentiation. DNA was extracted from a paraffin-embedded cell block or frozen needle core fragments. Exon 19 deletions and the L858R mutation in exon 21 of EGFR were detected using PCR followed by capillary electrophoresis for fragment sizing. KRAS mutational analysis was performed by real-time PCR using a set of seven different Taqman(r) allelic discrimination assays to detect six mutations in codon 12 and one mutation in codon 13. Six cases (12%) showed EGFR mutations, 12 (24%) showed KRAS mutations, and 38 (62%) contained neither EGFR nor KRAS mutations. The majority of patients had stage IV disease (78%); 20 samples (40%) were from metastatic sites. The presence of prominent INCI (P = 0.036), papillary fragments (P = 0.041), and histologic features of BAC on paraffin block (P = 0.039) correlated with the presence of EGFR mutations. The presence of necrosis (P = 0.030), squamoid features (P = 0.048), and poorly differentiated tumors (P = 0.025) were more likely to be identified in the KRAS positive group. |
| File Format | HTM / HTML |
| ISSN | 87551039 |
| Issue Number | 1 |
| Volume Number | 41 |
| e-ISSN | 10970339 |
| Journal | Diagnostic Cytopathology |
| Language | English |
| Publisher | Wiley-Liss |
| Publisher Date | 2013-01-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Cell Biology Discipline Pathology Adenocarcinoma Pathology Lung Neoplasms Mutation Proto-oncogene Proteins Genetics Receptor, Epidermal Growth Factor Ras Proteins Secondary Aged Cell Nucleolus Cell Size Dna, Neoplasm Exons Female Humans Male Middle Aged Necrosis Proto-oncogene Proteins P21(ras) Retrospective Studies Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Histology Pathology and Forensic Medicine |
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