NDLI: Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.

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Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.

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Establishing targeted carp TLR22 gene disruption via homologous recombination using CRISPR/Cas9.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chakrapani, Vemulawada Patra, Swagat Kumar Panda, Rudra Prasanna Rasal, Kiran Dashrath Jayasankar, Pallipuram Barman, Hirak Kumar
Description	Country affiliation: India Author Affiliation: Chakrapani V ( Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, Odisha, India.); Patra SK ( Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, Odisha, India.); Panda RP ( Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, Odisha, India.); Rasal KD ( Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, Odisha, India.); Jayasankar P ( Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, Odisha, India.); Barman HK ( Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar 751 002, Odisha, India. Electronic address: Hirak.Barman@icar.gov.in.)
Abstract	Recent advances in gene editing techniques have not been exploited in farmed fishes. We established a gene targeting technique, using the CRISPR/Cas9 system in Labeo rohita, a farmed carp (known as rohu). We demonstrated that donor DNA was integrated via homologous recombination (HR) at the site of targeted double-stranded nicks created by CRISPR/Cas9 nuclease. This resulted in the successful disruption of rohu Toll-like receptor 22 (TLR22) gene, involved in innate immunity and exclusively present in teleost fishes and amphibians. The null mutant, thus, generated lacked TLR22 mRNA expression. Altogether, this is the first evidence that the CRISPR/Cas9 system is a highly efficient tool for targeted gene disruption via HR in teleosts for generating model large-bodied farmed fishes.
File Format	HTM / HTML
ISSN	0145305X
Journal	Developmental & Comparative Immunology
Volume Number	61
e-ISSN	18790089
Language	English
Publisher	Elsevier
Publisher Date	2016-08-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Immunology

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