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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Charalambous, Christoforos C. Matsopoulos, George K. |
| Description | Country affiliation: Greece Author Affiliation: Charalambous CC ( School of Electrical and Computer Engineering, National Technical University of Athens, Greece.) |
| Abstract | In this paper, a new methodological scheme for the gridding of DNA microarrays is proposed. The scheme composes of a series of processes applied sequentially. Each DNA microarray image is pre-processed to remove any noise and the center of each spot is detected using a template matching algorithm. Then, an initial gridding is automatically placed on the DNA microarray image by 'building' rectangular pyramids around the detected spots' centers. The gridlines 'move' between the pyramids, horizontally and vertically, forming this initial grid. Furthermore, a refinement process is applied composing of a five-step approach in order to correct gridding imperfections caused by its initial placement, both in non-spot cases and in more than one spot enclosure cases. The proposed gridding scheme is applied on DNA microarray images under known transformations and on real-world DNA data. Its performance is compared against the projection pursuit method, which is often used due to its speed and simplicity, as well as against a state-of-the-art method, the Optimal Multi-level Thresholding Gridding (OMTG). According to the obtained results, the proposed gridding scheme outperforms both methods, qualitatively and quantitatively. |
| File Format | HTM / HTML |
| ISSN | 00104825 |
| Issue Number | 10 |
| Volume Number | 43 |
| e-ISSN | 18790534 |
| Journal | Computers in Biology and Medicine |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biology Discipline Biomedical Informatics Computational Biology Methods Dna Chemistry Image Processing, Computer-assisted Oligonucleotide Array Sequence Analysis Algorithms Genetics Databases, Genetic Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health Informatics Computer Science Applications |
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