NDLI: CO2 induced seawater acidification impacts sea urchin larval development II: gene expression patterns in pluteus larvae.

Please wait, while we are loading the content...

The interactive effects of a gradual temperature decrease and long-term food deprivation on cardiac and hepatic blood flows in rainbow trout (Oncorhynchus mykiss).

CO2 induced seawater acidification impacts sea urchin larval development II: gene expression patterns in pluteus larvae.

CO2 induced seawater acidification impacts sea urchin larval development I: elevated metabolic rates decrease scope for growth and induce developmental delay.

Production of digestive enzymes along the gut of the giant keyhole limpet Megathura crenulata (Mollusca: Vetigastropoda).

Is there an influence of body mass on digesta mean retention time in herbivores? A comparative study on ungulates.

Analysis of glucose metabolism in farmed European sea bass (Dicentrarchus labrax L.) using deuterated water.

Changes in body fluids of the cocooning fossorial frog Cyclorana australis in a seasonally dry environment.

Targeted gene expression profiling in European sea bass (Dicentrarchus labrax, L.) follicles from primary growth to late vitellogenesis.

Hypoxic alligator embryos: chronic hypoxia, catecholamine levels and autonomic responses of in ovo alligators.

Effects of predictable and unpredictable food restriction on the stress response in molting and non-molting European starlings (Sturnus vulgaris).

Morphological and biochemical evidence for the evolution of salt glands in snakes.

Is water uptake by reptilian eggs regulated by physiological processes of embryos or a passive hydraulic response to developmental environments?

Effect of environmental temperature on growth- and reproduction-related hormones gene expression in the female blue gourami (Trichogaster trichopterus).

Digestive enzyme activity of two stonefly species (Insecta, Plecoptera) and their feeding habits.

Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity.

Year: 2011 Volume: 160 Issue: 2

Year: 2011 Volume: 160 Issue: 1

Year: 2011 Volume: 159

Year: 2011 Volume: 158

Year: 2010 Volume: 157

Year: 2010 Volume: 156

Year: 2010 Volume: 155

Year: 2009 Volume: 154

Year: 2009 Volume: 153

Year: 2009 Volume: 152

Year: 2008 Volume: 151

Year: 2008 Volume: 150

Year: 2008 Volume: 149

CO2 induced seawater acidification impacts sea urchin larval development II: gene expression patterns in pluteus larvae.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Stumpp, M. Dupont, S. Thorndyke, M. C. Melzner, F.
Description	Country affiliation: Germany Author Affiliation: Stumpp M ( Biological Oceanography, Leibniz Institute of Marine Sciences (IFM-GEOMAR), Kiel, Germany. mstumpp@ifm-geomar.de)
Abstract	Extensive use of fossil fuels is leading to increasing CO(2) concentrations in the atmosphere and causes changes in the carbonate chemistry of the oceans which represents a major sink for anthropogenic CO(2). As a result, the oceans' surface pH is expected to decrease by ca. 0.4 units by the year 2100, a major change with potentially negative consequences for some marine species. Because of their carbonate skeleton, sea urchins and their larval stages are regarded as likely to be one of the more sensitive taxa. In order to investigate sensitivity of pre-feeding (2 days post-fertilization) and feeding (4 and 7 days post-fertilization) pluteus larvae, we raised Strongylocentrotus purpuratus embryos in control (pH 8.1 and pCO(2) 41 Pa e.g. 399 µatm) and CO(2) acidified seawater with pH of 7.7 (pCO(2) 134 Pa e.g. 1318 µatm) and investigated growth, calcification and survival. At three time points (day 2, day 4 and day 7 post-fertilization), we measured the expression of 26 representative genes important for metabolism, calcification and ion regulation using RT-qPCR. After one week of development, we observed a significant difference in growth. Maximum differences in size were detected at day 4 (ca. 10% reduction in body length). A comparison of gene expression patterns using PCA and ANOSIM clearly distinguished between the different age groups (two-way ANOSIM: Global R=1) while acidification effects were less pronounced (Global R=0.518). Significant differences in gene expression patterns (ANOSIM R=0.938, SIMPER: 4.3% difference) were also detected at day 4 leading to the hypothesis that differences between CO(2) treatments could reflect patterns of expression seen in control experiments of a younger larva and thus a developmental artifact rather than a direct CO(2) effect. We found an up regulation of metabolic genes (between 10%and 20% in ATP-synthase, citrate synthase, pyruvate kinase and thiolase at day 4) and down regulation of calcification related genes (between 23% and 36% in msp130, SM30B, and SM50 at day 4). Ion regulation was mainly impacted by up regulation of Na(+)/K(+)-ATPase at day 4 (15%) and down regulation of NHE3 at day 4 (45%). We conclude that in studies in which a stressor induces an alteration in the speed of development, it is crucial to employ experimental designs with a high time resolution in order to correct for developmental artifacts. This helps prevent misinterpretation of stressor effects on organism physiology.
File Format	HTM / HTML
ISSN	10956433
Issue Number	3
Volume Number	160
e-ISSN	15314332
Journal	Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Language	English
Publisher	Elsevier
Publisher Date	2011-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Physiology Discipline Molecular Biology Carbon Dioxide Toxicity Carbonic Acid Chemistry Environmental Pollution Gene Expression Profiling Sea Urchins Growth & Development Genetics Animals Humans Hydrogen-ion Concentration Larva Seawater Up-regulation Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physiology Molecular Biology Biochemistry Aquatic Science Animal Science and Zoology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

CO2 induced seawater acidification impacts sea urchin larval development I: elevated metabolic rates decrease scope for growth and induce developmental delay.

Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification

Biogenic acidification reduces sea urchin gonad growth and increases susceptibility of aquaculture to ocean acidification.

Assessing physiological tipping point of sea urchin larvae exposed to a broad range of pH.

Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification.

Effects of ocean-acidification-induced morphological changes on larval swimming and feeding.

Vulnerability of the calcifying larval stage of the Antarctic sea urchin Sterechinus neumayeri to near-future ocean acidification and warming.

Impacts of ocean acidification on development of the meroplanktonic larval stage of the sea urchin Centrostephanus rodgersii

Analysis of Pacific oyster larval proteome and its response to high-CO2.

CO2 induced seawater acidification impacts sea urchin larval development II: gene expression patterns in pluteus larvae.

Similar Documents

CO2 induced seawater acidification impacts sea urchin larval development I: elevated metabolic rates decrease scope for growth and induce developmental delay.

Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification

Biogenic acidification reduces sea urchin gonad growth and increases susceptibility of aquaculture to ocean acidification.

Assessing physiological tipping point of sea urchin larvae exposed to a broad range of pH.

Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification.

Effects of ocean-acidification-induced morphological changes on larval swimming and feeding.

Vulnerability of the calcifying larval stage of the Antarctic sea urchin Sterechinus neumayeri to near-future ocean acidification and warming.

Impacts of ocean acidification on development of the meroplanktonic larval stage of the sea urchin Centrostephanus rodgersii

Analysis of Pacific oyster larval proteome and its response to high-CO2.

CO2 induced seawater acidification impacts sea urchin larval development II: gene expression patterns in pluteus larvae.