NDLI: PCR detection of Streptococcus mutans and Aggregatibacter actinomycetemcomitans in dental plaque samples from Haitian adolescents.

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PCR detection of Streptococcus mutans and Aggregatibacter actinomycetemcomitans in dental plaque samples from Haitian adolescents.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Psoter, Walter J. Ge, Yao Russell, Stefanie L. Chen, Zhou Katz, Ralph V. Jean-Charles, Germain Li, Yihong
Spatial Coverage	Haiti
Description	Country affiliation: United States Author Affiliation: Psoter WJ ( Department of Epidemiology and Health Promotion, New York University College of Dentistry, 345 E. 24th Street, New York, NY 10010-4086, USA.)
Abstract	Streptococcus mutans and Aggregatibacter actinomycetemcomitans are oral pathogens associated with dental caries and periodontitis, respectively. The aim of this study was to determine the colonization of these two microorganisms in the dental plaque of a group of Haitian adolescents using two different polymerase chain reaction (PCR) methods, standard PCR, and quantitative real-time PCR (qPCR) assays. Fifty-four pooled supra-gingival plaque samples and 98 pooled sub-gingival plaque samples were obtained from 104 12- to19-year-old rural-dwelling Haitians. The total genomic DNA of bacteria was isolated from these samples, and all participants also received caries and periodontal examinations. Caries prevalence was 42.2%, and the mean decayed, missing, and filled surface (DMFS) was 2.67 ± 5.3. More than half of the adolescents (53.3%) experienced periodontal pockets (Community Periodontal Index score ≥3). S. mutans was detected in 67.3% by qPCR and 38.8% by PCR of the supra-gingival plaque samples (p < 0.01), and 36.6% by qPCR and 8.1% by PCR of the sub-gingival samples (p < 0.01). A. actinomycetemcomitans was detected in 85.1% by qPCR and 44.0% by PCR of the sub-gingival samples (p < 0.01), but the prevalence was similar, 67.3% by qPCR and 59.2% by PCR, in the supra-gingival plaque samples. Neither age nor gender was significantly correlated to the bacterial colonization. The results demonstrated a moderate-to-high prevalence of S. mutans and A. actinomycetemcomitans in the Haitian adolescent population, and qPCR is more sensitive than standard PCR in field conditions. These findings suggest that qPCR should be considered for field oral epidemiologic studies and may be necessary in investigations having major logistic challenges.
File Format	HTM / HTML
ISSN	14326981
e-ISSN	14363771
DOI	10.1007/s00784-010-0413-y
Journal	Clinical Oral Investigations
Issue Number	4
Volume Number	15
Language	English
Publisher	Springer
Publisher Date	2011-08-01
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Discipline Dentistry Discipline General Surgery Aggregatibacter Actinomycetemcomitans Isolation & Purification Dental Plaque Microbiology Polymerase Chain Reaction Streptococcus Mutans Adolescent Dmf Index Dna, Bacterial Dental Caries Classification Dental Restoration, Permanent Statistics & Numerical Data Gingival Pocket Haiti Periodontal Index Periodontal Pocket Rural Health Tooth Loss Comparative Study Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Dentistry

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