NDLI: Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

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Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

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Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wan, Qian Lu, Hua Wu, Lin-Tao Liu, Xia Xiang, Jun-Bei
Description	Author Affiliation: Wan Q ( The Second Affiliated Medical College and Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610041, P. R. China.)
Abstract	Retinoic acid (RA) and oleanolic acid (OA) were studied about their potential to induce mouse embryonic stem cell R1/E (MESC-R1/E) to differentiate toward germ cells. Embryoid bodies (EBs) first formed from MESC-R1/E and EBs were allowed to attach to the bottoms of normal cell-culturing plate and grow. Then, different compounds including RA, OA and so on were respectively added to induce MESC-R1/E to differentiate. After 72 h, microscopy images were taken for all interventions, then total RNAs were extracted, cDNAs were synthesized and real-time fluorescence quantitative PCR (qPCR) was performed to detect the transcriptional expression patterns of 11 reproductive-differentiation-related genes for different compounds respectively. During the data analysis, it was found RA significantly up-regulated the expression levels of GDF-9, Stra8, SCP3, Mvh, ZP1, ZP2, and ZP3, while significantly down-regulated the levels of Itag6 and Itgb1, and the level of Oct-4 was down-regulated insignificantly, while the level of TP2 was up-regulated insignificantly; OA significantly up-regulated the expression levels of Stra8, SCP3, Mvh, ZP1, ZP2, Itgb1, and TP2, and the levels of Oct-4, GDF-9, ZP3, and Itga6 were up-regulated insignificantly. The data showed that RA can induce MESC-R1/E to differentiate toward female germ cells while OA can induce MESC-R1/E to differentiate toward male and female germ cells.
File Format	HTM / HTML
ISSN	10656995
Issue Number	12
Volume Number	38
e-ISSN	10958355
Journal	Cell Biology International
Language	English
Publisher	Wiley
Publisher Date	2014-12-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Cell Biology Cell Differentiation Drug Effects Embryonic Stem Cells Cytology Oleanolic Acid Pharmacology Ovum Tretinoin Animals Cells, Cultured Metabolism Female Mice Up-regulation Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine

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Retinoic acid can induce mouse embryonic stem cell R1/E to differentiate toward female germ cells while oleanolic acid can induce R1/E to differentiate toward both types of germ cells.