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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Vondran, Florian Wolfgang Rudolf Katenz, Ekaterina Schwartlander, Ruth Morgul, Mehmet Haluk Raschzok, Nathanael Gong, Xiaobing Cheng, Xiangdong Kehr, Daniel Sauer, Igor Maximilian |
| Description | Country affiliation: Germany Author Affiliation: Vondran FW ( Department of General, Visceral, and Transplantation Surgery, Charité Universitätsmedizin Berlin, Campus Virchow, Berlin, Germany. florian@vondran.org) |
| Abstract | Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two-step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma-glutamyl-transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties. |
| File Format | HTM / HTML |
| ISSN | 0160564X |
| Issue Number | 3 |
| Volume Number | 32 |
| e-ISSN | 15251594 |
| Journal | Artificial Organs |
| Language | English |
| Publisher | Wiley-Blackwell |
| Publisher Date | 2008-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Transplantation Cell Separation Hepatectomy Hepatocytes Pathology Liver Adult Aged Albumins Biosynthesis Aspartate Aminotransferases Metabolism Cell Culture Techniques Cell Shape Cell Survival Cells, Cultured Female Enzymology Humans L-lactate Dehydrogenase Surgery Male Middle Aged Protein Biosynthesis Time Factors Urea Gamma-glutamyltransferase Blood Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomaterials Bioengineering Biomedical Engineering |
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