NDLI: Effects of the Hydration State on the Mid-Infrared Spectra of Urea and Creatinine in Relation to Urine Analyses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oliver, Katherine V. Maréchal, Amandine Rich, Peter R.
Description	Country affiliation: United kingdom Author Affiliation: Oliver KV ( Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, United Kingdom.); Maréchal A ( Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, United Kingdom.); Rich PR ( Glynn Laboratory of Bioenergetics, Institute of Structural and Molecular Biology, University College London, United Kingdom prr@ucl.ac.uk.)
Abstract	When analyzing solutes by Fourier transform infrared (FT-IR) spectroscopy in attenuated total reflection (ATR) mode, drying of samples onto the ATR crystal surface can greatly increase solute band intensities and, therefore, aid detection of minor components. However, analysis of such spectra is complicated by the existence of alternative partial hydration states of some substances that can significantly alter their infrared signatures. This is illustrated here with urea, which is a dominant component of urine. The effects of hydration state on its infrared spectrum were investigated both by incubation in atmospheres of fixed relative humidities and by recording serial spectra during the drying process. Significant changes of absorption band positions and shapes were observed. Decomposition of the CN antisymmetric stretching (νas) band in all states was possible with four components whose relative intensities varied with hydration state. These correspond to the solution (1468 cm(-1)) and dry (1464 cm(-1)) states and two intermediate (1454 cm(-1) and 1443 cm(-1)) forms that arise from specific urea-water and/or urea-urea interactions. Such intermediate forms of other compounds can also be formed, as demonstrated here with creatinine. Recognition of these states and their accommodation in analyses of materials such as dried urine allows more precise decomposition of spectra so that weaker bands of diagnostic interest can be more accurately defined.
File Format	HTM / HTML
ISSN	00037028
Issue Number	6
Journal	Applied Spectroscopy
Volume Number	70
e-ISSN	19433530
Language	English
Publisher	Sage Publication
Publisher Date	2016-06-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Imaging Discipline Analytical Chemistry Discipline Radiology
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Instrumentation

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