NDLI: Genus-specific primers targeting the 16S rRNA gene for PCR detection of members of the genus Verrucosispora.

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Genus-specific primers targeting the 16S rRNA gene for PCR detection of members of the genus Verrucosispora.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Xie, Qingyi Hong, Kui Goodfellow, Michael
Description	Author Affiliation: Xie Q ( Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, People's Republic of China.)
Abstract	Little is known about the genus Verrucosispora though it does contain organisms which produce novel antibiotics. A set of genus-specific oligonucleotide primers was generated to gain an insight into the presence, distribution and taxonomic diversity of members of this genus in diverse samples taken from marine habitats. In silico and pure culture studies showed that the primers matched perfectly with target sequences of the 16S rRNA genes of representatives of the genus Verrucosispora. The primers, designated S-G-Verr-0195-a-S-20 and S-G-Verr-1152-a-A-18, amplified an ≈960 bp stretch of the 16S rRNA genes of Verrucosispora strains but not those of representatives of other genera classified in the family Micromonosporaceae. Genus-specific amplicons were detected from 17 out of 20 community DNA samples prepared from diverse marine sediments and coastal soils. Phylogenetic analysis of over 40% of clones derived from five of the samples indicated they belonged to novel Verrucosispora species. The primers were also used to confirm the identity of Verrucosispora-like strains isolated from two of the environmental samples. The primers can be used to facilitate the isolation of novel Verrucosispora strains by allowing prescreening of environmental samples and the subsequent identification of verrucosisporae on selective isolation plates. For this purpose, a novel medium facilitating the recovery of Verrucosispora strains was formulated and used to recover novel isolates validated using the novel PCR primers. This medium may be useful as the basis for development of a selective medium.
File Format	HTM / HTML
ISSN	00036072
Issue Number	1
Volume Number	100
e-ISSN	15729699
Journal	Antonie van Leeuwenhoek
Language	English
Publisher	Springer
Publisher Date	2011-06-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Immunology Dna Primers Genetics Micromonosporaceae Isolation & Purification Polymerase Chain Reaction Instrumentation Rna, Ribosomal, 16s Dna, Bacterial Dna, Ribosomal Geologic Sediments Microbiology Classification Molecular Sequence Data Methods Soil Microbiology Species Specificity Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Microbiology

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