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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nie, Guo-Xing Ming, Hong Wei, Da-Qiao Zhou, En-Min Tang, Xia Cheng, Juan Tang, Shu-Kun Li, Wen-Jun |
| Spatial Coverage | China |
| Description | Author Affiliation: Nie GX ( College of Life Sciences, Henan Normal University, Xinxiang, People's Republic of China.) |
| Abstract | A novel Gram-stain positive, aerobic, non-motile, spore-forming actinobacterium, designated YIM 75926(T), was isolated from a soil sample collected at soil forest in Yuanmo county of Yunnan province, south-west China. Its taxonomic position was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain YIM 75926(T) belongs to the genus Pseudonocardia and was closely related to Pseudonocardia halophobica DSM 43089(T) (98.1% similarity). Strain YIM 75926(T) had MK-8 (H(4)) as the predominant menaquinone. The whole organism hydrolysates mainly consisted of meso-diaminopimelic acid, mannose, glucose, galactose and arabinose. The major cellular fatty acids were iso-C(16:0) (37.16%) and C(16:0) (12.43%). The DNA G+C content of strain YIM 75926(T) was 70.6 mol%. The resultant phylogenetic trees further showed that strain YIM 75926(T) belong to Pseudonocardia and had a distinct subclade within the evolutionary radiation of the genus Pseudonocardia. On the basis of its comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 75926(T) represent a novel species of the genus Pseudonocardia, named Pseudonocardia yuanmoensis sp. nov. The type strain is YIM 75926(T) (=CCTCC AA 2011017(T) = JCM 18055(T)). |
| File Format | HTM / HTML |
| ISSN | 00036072 |
| Issue Number | 4 |
| Volume Number | 101 |
| e-ISSN | 15729699 |
| Journal | Antonie van Leeuwenhoek |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-05-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Immunology Actinomycetales Classification Isolation & Purification Soil Microbiology Chemistry Genetics Bacterial Typing Techniques Base Composition China Cluster Analysis Dna, Bacterial Dna, Ribosomal Fatty Acids Analysis Molecular Sequence Data Phylogeny Rna, Ribosomal, 16s Sequence Analysis, Dna Spores, Bacterial Cytology Trees Vitamin K 2 Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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