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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Evans, Tegan N. Watson, Garth Rees, Gavin N. Seviour, Robert J. |
| Description | Author Affiliation: Evans TN ( La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, VIC, 3086, Australia, tnevans1984@gmail.com.) |
| Abstract | We compared the relative values of denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) for profiling fungal communities in wastewater treatment plants using both ITS and 18S rRNA gene fragments as phylogenetic markers. A similar number of fungal ribotypes was obtained with both methods for the same treatment plant when the ITS primer set was used, while a greater number of ribotypes was obtained with T-RFLP compared to DGGE with the 18S rRNA primer set. Non-metric multi-dimensional scaling of presence/absence data and analysis of similarity showed that both methods could distinguish between the different plant communities at a statistically significant level (p < 0.05), regardless of which phylogenetic marker was used. The data suggest that both methods can be used preferably together to profile activated sludge fungal communities. A comparison of profiles generated with both these phylogenetic markers based on the number of ribotypes/bands, suggests that the 18S rRNA region is more discriminatory than the ITS region. Detected differences in fungal community compositions between plants probably reflect differences in their influent compositions and operational parameters. |
| File Format | HTM / HTML |
| ISSN | 00036072 |
| Issue Number | 3 |
| Volume Number | 105 |
| e-ISSN | 15729699 |
| Journal | Antonie van Leeuwenhoek |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2014-03-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Immunology Biodiversity Fungi Classification Sewage Microbiology Dna, Ribosomal Spacer Genetics Denaturing Gradient Gel Electrophoresis Polymorphism, Restriction Fragment Length Rna, Ribosomal, 18s Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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