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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fernandes, Tatiana Alves Rigamonte da Silveira, Wendel Batista Passos, Flávia Maria Lopes Zucchi, Tiago Domingues |
| Description | Author Affiliation: Fernandes TA ( Laboratório de Microbiologia Ambiental, Embrapa Meio Ambiente, Rodovia SP 340, Km 127,5, CP 69, Jaguariúna, SP, 13820-000, Brazil, tatirigamonte@yahoo.com.br.) |
| Abstract | Although many putative laccase-like genes have been assigned to members of the phylum Actinobacteria, few of the related enzymes have been characterized so far. It is noteworthy, however, that this small number of enzymes has presented properties with industrial relevance. This observation, combined with the recognized biotechnological potential and the capability of this phylum to degrade recalcitrant soil polymers, has attracted attention for bioprospective approaches. In the present work, we have designed and tested primers that were specific for detection of sub-groups of laccase-like genes within actinomycetes, which corresponded to the superfamilies I and K from the classification presented by the laccase and multicopper oxidase engineering database. The designed primers have amplified laccase-like gene fragments from actinomycete isolates that were undetectable by primers available from the literature. Furthermore, phylogenetic alignments suggest that some of these fragments may belong to new laccases-like proteins, and thus emphasize the benefits of designing subgroup-specific primers. |
| File Format | HTM / HTML |
| ISSN | 00036072 |
| Issue Number | 2 |
| Volume Number | 106 |
| e-ISSN | 15729699 |
| Journal | Antonie van Leeuwenhoek |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2014-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Immunology Actinobacteria Enzymology Dna Primers Genetics Laccase Cluster Analysis Dna, Bacterial Chemistry Dna, Ribosomal Molecular Sequence Data Phylogeny Polymerase Chain Reaction Rna, Ribosomal, 16s Sequence Alignment Sequence Analysis, Dna Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Microbiology |
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