| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wu, Zhuangyuan Zheng, Xinbiao Luo, Yongming Huo, Fei Dong, Hong Zhang, Guoting Yu, Weihao Tian, Fang He, Liangjun Chen, Jingbo |
| Description |
Country affiliation: China Author Affiliation: Wu Z ( College of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, China.); Zheng X ( Institute of Animal Science, Xinjiang Academy of Animal Science, Urumqi 830000, Xinjiang, China.); Luo Y ( Institute of Animal Science, Xinjiang Academy of Animal Science, Urumqi 830000, Xinjiang, China.); Huo F ( Institute of Animal Science, Xinjiang Academy of Animal Science, Urumqi 830000, Xinjiang, China.); Dong H ( Institute of Animal Science, Xinjiang Academy of Animal Science, Urumqi 830000, Xinjiang, China.); Zhang G ( Institute of Animal Science, Xinjiang Academy of Animal Science, Urumqi 830000, Xinjiang, China.); Yu W ( The Breeding Center of Yili Horse, Zhaosu 835600, Xingjiang, China.); Tian F ( College of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, China.); He L ( College of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, China.); Chen J ( College of Animal Science and Technology, Shihezi University, Shihezi 832000, Xinjiang, China) |
| Abstract |
The present study investigates the effects of five cryoprotectants (CPAs) and cryoprotectant combinations on the post-thaw total motility, progressive motility, viability, mitochondrial membrane potential and acrosome integrity in stallion spermatozoa. In Experiment 1, the objective was to compare the impact of different concentrations (2.5%, 3.5% and 5%) of a single CPA, including glycerol (Gly), ethylene glycol (EG), dimethyl sulphoxide (DMSO), methyl formamide (MF), and dimethylformamide (DMF) for stallion spermatozoa cryopreservation. In Experiment 2, two or more CPAs were used to assess whether this improved post-thaw spermatozoa quality. Gly, MF and DMF, were used to prepare seven combinations of freezing extender with different mixtures of cryoprotectant, and the 3.5% Gly, MF and DMF were used as a control group. The results show that post-thaw total motility, progressive motility, viability, and mitochondrial membrane potential for all concentrations of EG and DMSO were less than the 3.5% and 5% Gly and MF and 2.5% and 3.5% DMF (P<0.05). Use of the 3.5% concentration resulted in the greater post-thaw total motility and progressive motility than the 2.5% and 5% concentrations for all CPAs. The results for the use of different combinations of cryoprotectant indicate there are differences in progressive motility and viability. The viability with the use of Gly(2/3)+MF(1/3) was 44.65% and was greater than the Gly(1/3)+MF(1/3)+DMF(1/3) (30.96%), MF(2/3)+DMF(1/3) (35.05%), Gly (32.21%) and MF(33.76%) (P<0.05). The progressive motility with the use of the MF(2/3)+Gly(1/3) combination was 36.0% and was greater than in the DMF (25.0%) and MF(2/3)+DMF(1/3) (22.7%) (P<0.05). These results suggest that using the appropriate cryoprotectant combination instead of a single cryoprotectant can improve horse spermatozoa cryopreservation. |
| File Format |
HTM / HTML |
| ISSN |
03784320 |
| Volume Number |
163 |
| e-ISSN |
18732232 |
| Journal |
Animal Reproduction Science |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-12-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Animal science Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Cryopreservation Veterinary Cryoprotective Agents Pharmacology Horses Physiology Semen Preservation Spermatozoa Animals Methods Administration & Dosage Male Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Animal Science and Zoology Endocrinology Food Animals |
