| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Santos, Gustavo Martins Gomes Dos Silva-Santos, Katia Cristina Barreiros, Thales Ricardo Rigo Morotti, Fábio Sanches, Bruno Valente de Moraes, Fábio Lucas Zito Blaschi, Wanessa Seneda, Marcelo Marcondes |
| Description |
Country affiliation: Brazil Author Affiliation: Santos GM ( Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina 86051-990, PR, Brazil.); Silva-Santos KC ( Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina 86051-990, PR, Brazil.); Barreiros TR ( Laboratório de Reprodução Animal, Universidade Estadual do Norte do Paraná (Uenp), Bandeirantes 86360-000, PR, Brazil.); Morotti F ( Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina 86051-990, PR, Brazil.); Sanches BV ( Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina 86051-990, PR, Brazil.); de Moraes FL ( Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina 86051-990, PR, Brazil.); Blaschi W ( Laboratório de Reprodução Animal, Universidade Estadual do Norte do Paraná (Uenp), Bandeirantes 86360-000, PR, Brazil.); Seneda MM ( Laboratório de Biotecnologia da Reprodução Animal (ReproA), DCV-CCA-Universidade Estadual de Londrina (UEL), Londrina 86051-990, PR, Brazil. Electronic address: mseneda@uel.br.) |
| Abstract |
The objective was to compare the conception rates for FTAI and in vitro embryo production between Nelore cows with different antral follicle counts (AFC=number of follicles ≤3mm in diameter in the ovaries). Nelore cows (n=547) were subjected to ovulation synchronization. Randomly during the estrous cycle (D0), cows received an intravaginal device containing 1.9g P4 (CIDR(®)) and 2mg BE (Estrogin(®)), IM. When the device was removed (D8), the cows received 500µg PGF2 (Ciosin(®)), 300IU eCG (Novormon(®)) and 1mg EC (ECP(®)), IM. All cows were inseminated 48h after P4 device removal. Antral follicles ≥3mm were counted using an intravaginal microconvex transducer (D0), and the cows were assigned to high (G-High, ≥25 follicles, n=183), intermediate (G-Intermediate, 16-20 follicles, n=183) or low AFC groups (G-Low, ≤10 follicles, n=181). In another experiment, COCs were retrieved by OPU from Nelore cows (n=66), which were assigned to groups according to oocyte production: G-High (n=22, ≥40 oocytes), G-Intermediate (n=25, 18-25 oocytes) or G-Low (n=19, ≤7 oocytes). All COCs from the same cow were cultured individually (maximum of 25 COCs per drop) and then in vitro fertilized using thawed frozen sperm (2×10(8)/dose) from a Nelore sire of known fertility. The data were analyzed using a Kruskal-Wallis and a Chi-square test (P≤0.05). There was no difference in the conception rates after FTAI between Nelore cows with high, intermediate or low AFC (51.9 vs. 48.6 vs. 58.6%). The number of viable embryos was 18.4±6.7 (G-High), 6.1±3.6 (G-Intermediate) and 0.6±0.7 (G-Low; P<0.05). Therefore, AFC had no influence on the conception rates for FTAI; however, Nelore cows with high oocyte production exhibited better in vitro embryo production. |
| File Format |
HTM / HTML |
| ISSN |
03784320 |
| Volume Number |
165 |
| e-ISSN |
18732232 |
| Journal |
Animal Reproduction Science |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-02-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Animal science Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Cattle Physiology Embryo Culture Techniques Veterinary Fertilization Ovarian Follicle Animals Female Tissue And Organ Harvesting Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Animal Science and Zoology Endocrinology Food Animals |
