NDLI: UAE, MAE, SFE-CO2 and classical methods for the extraction of Mitragyna speciosa leaves.

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UAE, MAE, SFE-CO2 and classical methods for the extraction of Mitragyna speciosa leaves.

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UAE, MAE, SFE-CO2 and classical methods for the extraction of Mitragyna speciosa leaves.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Orio, Laura Alexandru, Lavinia Cravotto, Giancarlo Mantegna, Stefano Barge, Alessandro
Description	Country affiliation: Italy Author Affiliation: Orio L ( Dipartimento di Scienza e Tecnologia del Farmaco, Università di Torino, Via P Giuria 9, 10235 Torino, Italy.)
Abstract	Mitragyna speciosa, a tropical plant indigenous to Southeast Asia, is well known for its psychoactive properties. Its leaves are traditionally chewed by Thai and Malaysian farmers and manual labourers as it causes a numbing, stimulating effect. The present study aims to evaluate alkaloid yield and composition in the leaf extracts. For this purpose we have compared several non-conventional extraction techniques with classic procedures (room temperature or under heating). Dried M. speciosa leaves belonging to three batches of different origin (from Thailand, Malaysia and Indonesia) were extracted using ultrasound-assisted extraction (UAE), microwave-assisted extraction (MAE) and supercritical carbon dioxide extraction SFE-CO(2), using methanol, ethanol, water and binary mixtures. The extracts were compared using an HPLC/ESI-MS analysis of mitragynine and four other related alkaloids which were present in the alkaloid fraction. The extraction technique influences both the raw product yield and the relative alkaloid content of M. speciosa leaves. Of the several methods tested, MAE in a closed vessel at 110 °C (60 W, methanol/water 1:1) gave the highest alkaloid fraction amount, while UAE with an immersion horn at 25 °C (21.4 kHz, 50 W, methanol) showed the best yield for mitragynine. This work may prove to be a useful contribution to forensic, toxicological and pharmacognosy studies. Although the potential applications of M. speciosa alkaloids clearly need further investigation, these results may facilitate the scaling-up of their extraction.
File Format	HTM / HTML
ISSN	13504177
Issue Number	3
Volume Number	19
e-ISSN	18732828
Journal	Ultrasonics Sonochemistry
Language	English
Publisher	Elsevier
Publisher Date	2012-05-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Diagnostic Imaging Alkaloids Isolation & Purification Radiation Effects Mitragyna Chemistry Plant Extracts Plant Leaves Sonication Methods Chromatography, Supercritical Fluid High-energy Shock Waves Microwaves Radiation Dosage Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Environmental Chemistry Acoustics and Ultrasonics Radiology, Nuclear Medicine and Imaging Chemical Engineering Inorganic Chemistry

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