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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Meridor, David Gedanken, Aharon |
| Description | Country affiliation: Israel Author Affiliation: Meridor D ( Department of Chemistry and Kanbar Laboratory for Nanomaterials, Center for Advanced Materials and Nanotechnology, Bar-Ilan University, Ramat-Gan 52900, Israel.) |
| Abstract | Using high-intensity ultrasound, in situ generated -amylase nanoparticles (NPs) were immobilized on polyethylene (PE) films. The -amylase NP-coated PE films have been characterized by E-SEM, FTIR, DLS, XPS and RBS. The PE was reacted with HNO(3) and NPs of the -amylase were also deposited on the activated PE. The PE impregnated with -amylase (4 µg per 1mg PE) was used for hydrolyzing soluble potato starch to maltose. The immobilization improved the catalytic activity of -amylase at all the reaction conditions studied. The kinetic parameters, K(m) (5 and 4 g L(-1) for the regular and activated PE, respectively) and V(max) (5 × 10(-7) mol ml(-1) min(-1), almost the same numbers were obtained for the regular and activated PEs) for the immobilized amylase were found to slightly favor the respective values obtained for the free enzyme (K(m) = 6.6 g L(-1), V(max) = 3.7 × 10(-7) mol ml(-1) min(-1)). The enzyme remained bound to PE even after soaking the PE in a starch solution for 72 h and was still found to be weakly active. |
| File Format | HTM / HTML |
| ISSN | 13504177 |
| Issue Number | 1 |
| Volume Number | 20 |
| e-ISSN | 18732828 |
| Journal | Ultrasonics Sonochemistry |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-01-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Diagnostic Imaging Biocatalysis Enzymes, Immobilized Chemistry Metabolism Nanoparticles Polyethylene Alpha-amylases Drug Effects Hydrolysis Kinetics Nitric Acid Pharmacology Starch Ultrasonics Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Environmental Chemistry Acoustics and Ultrasonics Radiology, Nuclear Medicine and Imaging Chemical Engineering Inorganic Chemistry |
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