NDLI: Environmental friendly cold-mechanical/sonic enzymatic assisted extraction of genipin from genipap (Genipa americana).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ramos-de-la-Peña, Ana Mayela Renard, Catherine M. G. C. Wicker, Louise Montañez, Julio C. García-Cerda, Luis Alfonso Contreras-Esquivel, Juan Carlos
Description	Country affiliation: Mexico Author Affiliation: Ramos-de-la-Peña AM ( Laboratory of Applied Glycobiotechnology, Food Research Department, School of Chemistry, Universidad Autonoma de Coahuila, Saltillo City 25000, Coahuila State, Mexico.)
Abstract	An efficient cold-mechanical/sonic-assisted extraction technique was developed for extraction of genipin from genipap (Genipa americana) peel. Ultrasound assisted extraction (285 W, 24 kHz) was performed at 5, 10 and 15 °C for 5, 10 and 15 min. After cold-extraction, genipin was separated from pectin and proteins by aid of fungal pectinesterase. The maximum yield of non-cross-linked genipin was 7.85±0.33 mg/g, at 10 °C for 15 min by means of ultrasound extraction. The protein amount in extracts decreased in all samples. If mechanical process is combined with ultrasound assisted extraction the yield is increased by 8 times after the pectinesterase-assisted polyelectrolyte complex formation between pectic polysaccharides and proteins, avoiding the typical cross-linking of genipin. This novel process is viable to obtain non-cross-linked genipin, to be used as a natural colorant and cross-linker in the food and biotechnological industries.
File Format	HTM / HTML
ISSN	13504177
Issue Number	1
Volume Number	21
e-ISSN	18732828
Journal	Ultrasonics Sonochemistry
Language	English
Publisher	Elsevier
Publisher Date	2014-01-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Diagnostic Imaging Carboxylic Ester Hydrolases Metabolism Chemical Fractionation Methods Cold Temperature Iridoids Isolation & Purification Mechanical Phenomena Rubiaceae Chemistry Aspergillus Enzymology Green Chemistry Technology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Environmental Chemistry Acoustics and Ultrasonics Radiology, Nuclear Medicine and Imaging Chemical Engineering Inorganic Chemistry

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