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PCR test for detecting Taenia solium cysticercosis in pig carcasses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sreedevi, Chennuru Hafeez, Mohammad Kumar, Putcha Anand Rayulu, Vukka Chengalva Subramanyam, Kothapalli Venkata Sudhakar, Krovvidi
Spatial Coverage	India
Description	Country affiliation: India Author Affiliation: Sreedevi C ( Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, 517 502, Andhra Pradesh, India. sdevichennuru@rediffmail.com)
Abstract	Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.
File Format	HTM / HTML
ISSN	00494747
Issue Number	1
Volume Number	44
e-ISSN	15737438
Journal	Tropical Animal Health and Production
Language	English
Publisher	Springer
Publisher Date	2012-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Discipline Tropical Medicine Cysticercosis Veterinary Dna, Helminth Isolation & Purification Food Inspection Standards Meat Parasitology Polymerase Chain Reaction Methods Swine Diseases Diagnosis Taenia Solium Animals Epidemiology Cysticercus Genetics Food Parasitology India Limit Of Detection Sensitivity And Specificity Swine Journal Article Validation Studies
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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