NDLI: Prevalence and molecular characterization of Sarcocystis species in water buffaloes (Bubalus bubalus) in Egypt.

Please wait, while we are loading the content...

Feeding value of hays of tropical forage legumes in pigs: Vigna unguiculata, Psophocarpus scandens, Pueraria phaseoloides and Stylosanthes guianensis.

Cassava starch factory residues in the diet of slow-growing broilers.

Fattening ability and carcass value of Dhamari and Tehami ram lambs, $F_{1}$ crossbreds of Dhamari × Tehami in Yemen

Tick burden and prevalence of Theileria parva infection in Tarime zebu cattle in the lake zone of Tanzania.

Cystic hydatidosis in slaughtered goats from various municipal abattoirs in Oman.

Isolation and genetic characterization of Toxoplasma gondii from alpaca (Vicugna pacos) and sheep (Ovis aries).

Seroprevalence and molecular detection of Mycoplasma ovipneumoniae in goats in tropical China.

Molecular identification of chlamydial cause of abortion in small ruminants in Jordan.

Genetic and demographic bottleneck analysis of Indian camel breeds by microsatellite markers.

Short-wave solar radiation level willingly tolerated by lactating Holstein cows in an equatorial semi-arid environment.

Performance and meat quality of broiler chickens that are fed diets supplemented with Agaricus brasiliensis mushrooms.

Systematic review of the prevalence of paratuberculosis in cattle, sheep, and goats in Latin America and the Caribbean.

Effects of dairy husbandry practices and farm types on raw milk quality collected by different categories of dairy processors in the Peruvian Andes.

Occurrence and risk factors associated to Toxoplasma gondii infection in sheep from Rio de Janeiro, Brazil.

Prevalence of Mycobacterium bovis skin positivity and associated risk factors in cattle from western Uganda.

Marketing of meat sheep with intensive finishing in southern state of Mexico.

Short communication: estrus synchronization using progestogens or cloprostenol in tropical hair sheep.

Incidence of subclinical endometritis and its effects on reproductive performance of crossbred dairy cows.

Real-time PCR carried out on DNA extracted from serum or blood sample is not a good method for surveillance of bovine brucellosis.

Molecular investigation of foot-and-mouth disease virus in domestic bovids from Gharbia, Egypt.

Egg production response of laying chickens to feather clipping, cool water and aspirin during hot weather conditions.

Estrous and ovulatory responses following cervical artificial insemination in Thai-native goats given a new or once-used controlled internal drug release with human chorionic gonadotropin.

Effects of road transportation or droving on the weight and metabolism of young bulls.

Meta-analysis of Brucella seroprevalence in dairy cattle of Ethiopia.

Is the Santa Inês sheep a typical non-seasonal breeder in the Brazilian Southeast?

Dietary nano-chromium tripicolinate increases feed intake and decreases plasma cortisol in finisher gilts during summer

Genetic analysis of post-weaning growth traits of Thalli sheep under tropical conditions.

Effects of steam-treated rice straw feeding on growth, digestibility, and plasma volatile fatty acids of goats under different housing systems.

Sheep and goat production objectives in pastoral and agro-pastoral production systems in Chifra district of Afar, Ethiopia.

Year: 2014 Volume: 46 Issue: 7

Year: 2014 Volume: 46 Issue: 6

Year: 2014 Volume: 46 Issue: 5

Year: 2014 Volume: 46 Issue: 4

Year: 2014 Volume: 46 Issue: 3

Year: 2014 Volume: 46 Issue: 2

Year: 2014 Volume: 46 Issue: 1

Year: 2013 Volume: 45

Year: 2012 Volume: 44

Year: 2011 Volume: 43

Year: 2010 Volume: 42

Year: 2009 Volume: 41

Year: 2008 Volume: 40

Year: 1996 Volume: 28

Prevalence and molecular characterization of Sarcocystis species in water buffaloes (Bubalus bubalus) in Egypt.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ashmawy, Karam I. Abu-Akkada, Somaia S. Ghashir, Mohamed Bn
Spatial Coverage	Egypt
Description	Country affiliation: Egypt Author Affiliation: Ashmawy KI ( Parasitology Department, Faculty of Veterinary Medicine, Alexandria University, Edfina, Egypt.)
Abstract	The present study was planned to investigate the prevalence of Sarcocystis spp. among slaughtered water buffaloes (Bubalus bubalis) at Alexandria province, Egypt. Three hundred blood samples were collected from slaughtered buffaloes (5-7 years old). Two techniques were used to evaluate the seroprevalence of Sarcocystis spp., enzyme-linked immunosorbent assay (ELISA) and indirect haemagglutination assay (IHA). It was revealed that 203 (67.6 %) and 191 (63.6 %) of the tested serum samples were seropositive to Sarcocystis spp. by ELISA and IHA, respectively. The results of sensitivity and specificity of IHA relative to ELISA were 94 and 100 %, respectively. For molecular characterization of inter- and intra-species genetic polymorphism within Egyptian isolates of Sarcocystis spp. of water buffaloes, polymerase chain reaction (PCR) and polymerase chain reaction-restriction length polymorphisms (PCR-RFLPs) were performed on four macroscopic isolates. The isolates represented two different geographical regions of Egypt, Alexandria and Assuit provinces. Alexandria isolates (large and small-sized cyst of the same host) and Assuit isolates (large and small-sized cyst of the same host) were used. The 18S rDNA of the macroscopic cysts were characterized, in tandem, by four restriction endonucleases, RsaI, MboI, SspI and DraI. RsaI and MboI enzymes did not show any restriction sites for all isolates, leaving the amplified fragments without cutting. SspI showed two fragments in Alexandria and Assuit small-sized isolates cut by the enzyme at 600-700-bp fragments, while Alexandria and Assuit large-sized cysts amplicons were not digested by this enzyme. The fourth enzyme, DraI, cut the PCR product of Alexandria large-sized cysts into two fragments (420-780 bp), while Assuit large-sized amplicon was not cut. It could be concluded that there was a far distance between the two local isolates (small and large sized), but there were no differences between the large-sized isolates.
File Format	HTM / HTML
ISSN	00494747
Issue Number	8
Volume Number	46
e-ISSN	15737438
Journal	Tropical Animal Health and Production
Language	English
Publisher	Springer
Publisher Date	2014-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Discipline Tropical Medicine Sarcocystis Genetics Sarcocystosis Veterinary Animals Antigens, Protozoan Isolation & Purification Buffaloes Dna, Protozoan Egypt Epidemiology Enzyme-linked Immunosorbent Assay Polymerase Chain Reaction Methods Polymorphism, Restriction Fragment Length Prevalence Classification Parasitology Sensitivity And Specificity Seroepidemiologic Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Evidence for bovine besnoitiosis in Egypt-first serosurvey of Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt.

Detection of antibodies to Toxoplasma gondii in domesticated ruminants by recombinant truncated SAG2 enzyme-linked immunosorbent assay.

A new trivalent SnSAG surface antigen chimera for efficient detection of antibodies against Sarcocystis neurona and diagnosis of equine protozoal myeloencephalitis.

Prevalence and distribution patterns of Sarcocystis spp. in buffaloes in Beni-Suef, Egypt.

Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.

Infection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt

Seroprevalence of Toxoplasma gondii in wild kangaroos using an ELISA.

Seroprevalence and molecular characterization of Chlamydia abortus in frozen fetal and placental tissues of aborting ewes in northeastern Algeria.

A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays.

Prevalence and molecular characterization of Sarcocystis species in water buffaloes (Bubalus bubalus) in Egypt.

Similar Documents

Evidence for bovine besnoitiosis in Egypt-first serosurvey of Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt.

Detection of antibodies to Toxoplasma gondii in domesticated ruminants by recombinant truncated SAG2 enzyme-linked immunosorbent assay.

A new trivalent SnSAG surface antigen chimera for efficient detection of antibodies against Sarcocystis neurona and diagnosis of equine protozoal myeloencephalitis.

Prevalence and distribution patterns of Sarcocystis spp. in buffaloes in Beni-Suef, Egypt.

Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.

Infection survey, molecular, pathogenicity, and morphological characteristics of Sarcocystis species naturally infected water buffaloes (Bubalus bubalis) in Egypt

Seroprevalence of Toxoplasma gondii in wild kangaroos using an ELISA.

Seroprevalence and molecular characterization of Chlamydia abortus in frozen fetal and placental tissues of aborting ewes in northeastern Algeria.

A comparative study of Toxoplasma gondii seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays.

Prevalence and molecular characterization of Sarcocystis species in water buffaloes (Bubalus bubalus) in Egypt.