NDLI: Bone grafts engineered from human adipose-derived stem cells in perfusion bioreactor culture.

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Bone grafts engineered from human adipose-derived stem cells in perfusion bioreactor culture.

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Year: 2009 Volume: 15

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Bone grafts engineered from human adipose-derived stem cells in perfusion bioreactor culture.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fröhlich, Mirjam Grayson, Warren L. Marolt, Darja Gimble, Jeffrey M. Kregar-Velikonja, Nevenka Vunjak-Novakovic, Gordana
Description	Country affiliation: United States Author Affiliation: Fröhlich M ( Department of Biomedical Engineering, Columbia University, New York, New York, USA.)
Abstract	We report engineering of half-centimeter-sized bone constructs created in vitro using human adipose-derived stem cells (hASCs), decellularized bone scaffolds, and perfusion bioreactors. The hASCs are easily accessible, can be used in an autologous fashion, are rapidly expanded in culture, and are capable of osteogenic differentiation. hASCs from four donors were characterized for their osteogenic capacity, and one representative cell population was used for tissue engineering experiments. Culture-expanded hASCs were seeded on fully decellularized native bone scaffolds (4 mm diameter x 4 mm thick), providing the necessary structural and mechanical environment for osteogenic differentiation, and cultured in bioreactors with medium perfusion. The interstitial flow velocity was set to a level necessary to maintain cell viability and function throughout the construct volume (400 microm/s), via enhanced mass transport. After 5 weeks of cultivation, the addition of osteogenic supplements (dexamethasone, sodium-beta-glycerophosphate, and ascorbic acid-2-phosphate) to culture medium significantly increased the construct cellularity and the amounts of bone matrix components (collagen, bone sialoprotein, and bone osteopontin). Medium perfusion markedly improved the distribution of cells and bone matrix in engineered constructs. In summary, a combination of hASCs, decellularized bone scaffold, perfusion culture, and osteogenic supplements resulted in the formation of compact and viable bone tissue constructs.
File Format	HTM / HTML
ISSN	21524947
e-ISSN	21524955
DOI	10.1089/ten.TEA.2009.0164
Journal	Tissue Engineering Part A
Issue Number	1
Volume Number	16
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biotechnology Bioreactors Bone Substitutes Cell Differentiation Osteogenesis Subcutaneous Fat Cytology Tissue Engineering Cell Culture Techniques Cells, Cultured Perfusion Metabolism Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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