NDLI: Goat bone tissue engineering: comparing an intramuscular with a posterolateral lumbar spine location.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Van Gaalen, Steven M. Dhert, Wouter J. A. Kruyt, Moyo C. Yuan, Huipin Oner, F. Cumhur Van Blitterswijk, Clemens A. Verbout, Abraham J. de Bruijn, Joost D.
Description	Country affiliation: Netherlands Author Affiliation: van Gaalen SM ( Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands. s.m.gaalen@umcutrecht.nl)
Abstract	The aim of this study was to investigate the effect of implant location on bone formation in goats using autologous bone marrow-derived stromal cells in porous calcium phosphate scaffolds. Intramuscular locations were compared to posterolateral spine fusion locations in eight goats. As scaffolds, we used biphasic calcium phosphate porous blocks of 5 x 5 x 5 mm. Cell-seeded implants were compared to empty controls. Bone marrow-derived stromal cells were seeded at 8 million cells per cm(3) scaffold and cultured for 1 week. The follow-up time was 12 weeks. Fluorochromes were administered intravenously at 4, 6, and 8 weeks. Ectopic implants showed 21 +/- 3.6% bone formation for the cell seeded and 2.0 +/- 3.0% for the controls (p < 0.001). Paraspinal implants, however, showed 0.10 +/- 0.13% in the cell seeded compared to 0.023 +/- 0.027% in the control group (p = 0.09). A benefit of the cells was only found in the area closest to the paraspinal muscles (p < 0.01). Bone formation in the control samples was of later onset compared to the cell-seeded implants. In conclusion, cell-based bone tissue engineering in an ectopic environment was clearly effective. Similar constructs implanted in a posterolateral spine fusion location hardly showed any effect.
File Format	HTM / HTML
ISSN	21524947
Issue Number	2
Volume Number	16
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Implants, Experimental Lumbar Vertebrae Physiology Muscles Tissue Engineering Methods Animals Goats Imaging, Three-dimensional Injections, Intramuscular Stromal Cells Cytology Transplantation Time Factors Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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