NDLI: Shear stress induces nitric oxide-mediated vascular endothelial growth factor production in human adipose tissue mesenchymal stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bassaneze, Vinícius Barauna, Valério Garrone Lavini-Ramos, Carolina Kalil, Jorge Schettert, Isolmar Tadeu Miyakawa, Ayumi Aurea Krieger, José Eduardo
Description	Country affiliation: Brazil Author Affiliation: Bassaneze V ( Heart Institute InCor, University of São Paulo Medical School, São Paulo, Brazil.)
Abstract	It has been demonstrated that human adipose tissue-derived mesenchymal stem cells (hASCs) enhance vascular density in ischemic tissues, suggesting that they can differentiate into vascular cells or release angiogenic factors that may stimulate neoangiogenesis. Moreover, there is evidence that shear stress (SS) may activate proliferation and differentiation of embryonic and endothelial precursor stem cells into endothelial cells (ECs). In this work, we investigated the effect of laminar SS in promoting differentiation of hASCs into ECs. SS (10 dyn/cm(2) up to 96 h), produced by a cone plate system, failed to induce EC markers (CD31, vWF, Flk-1) on hASC assayed by RT-PCR and flow cytometry. In contrast, there was a cumulative production of nitric oxide (determined by Griess Reaction) and vascular endothelial growth factor (VEGF; by ELISA) up to 96 h of SS stimulation ( in nmol/10(4) cells: static: 0.20 +/- 0.03; SS: 1.78 +/- 0.38, n = 6; VEGF in pg/10(4) cells: static: 191.31 +/- v35.29; SS: 372.80 +/- 46.74, n = 6, P < 0.05). Interestingly, the VEGF production was abrogated by 5 mM N(G)-L-nitro-arginine methyl ester (L-NAME) treatment (VEGF in pg/10(4) cells: SS: 378.80 +/- 46.74, n = 6; SS + L-NAME: 205.84 +/- 91.66, n = 4, P < 0.05). The results indicate that even though SS failed to induce EC surface markers in hASC under the tested conditions, it stimulated NO-dependent VEGF production.
File Format	HTM / HTML
ISSN	15473287
Issue Number	3
Volume Number	19
e-ISSN	15578534
Journal	Stem Cells and Development
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Cell Biology Discipline Hematology Endothelial Cells Metabolism Mesenchymal Stromal Cells Nitric Oxide Vascular Endothelial Growth Factor A Adipose Tissue Cytology Adult Antigens, Cd31 Genetics Cell Differentiation Cells, Cultured Dose-response Relationship, Drug Enzyme Inhibitors Pharmacology Flow Cytometry Gene Expression Humans Drug Effects Middle Aged Ng-nitroarginine Methyl Ester Nitric Oxide Synthase Antagonists & Inhibitors Reverse Transcriptase Polymerase Chain Reaction Stress, Mechanical Time Factors Vascular Endothelial Growth Factor Receptor-2 Young Adult Von Willebrand Factor Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Hematology

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