NDLI: Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse.

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Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Davies, Timothy J. Fairchild, Paul J.
Description	Country affiliation: United kingdom Author Affiliation: Davies TJ ( Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.)
Abstract	The derivation of pluripotent embryonic stem cells (ESCs) from a variety of genetic backgrounds remains a desirable objective in the generation of mice functionally deficient in genes of interest and the modeling of human disease. Nevertheless, disparity in the ease with which different strains of mice yield ESC lines has long been acknowledged. Indeed, the generation of bona fide ESCs from the non obese diabetic (NOD) mouse, a well-characterized model of human type I diabetes, has historically proved especially difficult to achieve. Here, we report the development of protocols for the derivation of novel ESC lines from C57Bl/6 mice based on the combined use of high concentrations of leukemia inhibitory factor and serum-replacement, which is equally applicable to fresh and cryo-preserved embryos. Further, we demonstrate the success of this approach using Balb/K and CBA/Ca mice, widely considered to be refractory strains. CBA/Ca ESCs contributed to the somatic germ layers of chimeras and displayed a very high competence at germline transmission. Importantly, we were able to use the same protocol for the derivation of ESC lines from nonpermissive NOD mice. These ESCs displayed a normal karyotype that was robustly stable during long-term culture, were capable of forming teratomas in vivo and germline competent chimeras after injection into recipient blastocysts. Further, these novel ESC lines efficiently formed embryoid bodies in vitro and could be directed in their differentiation along the dendritic cell lineage, thus illustrating their potential application to the generation of cell types of relevance to the pathogenesis of type I diabetes.
File Format	HTM / HTML
ISSN	15473287
e-ISSN	15578534
DOI	10.1089/scd.2011.0427
Journal	Stem Cells and Development
Issue Number	10
Volume Number	21
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2012-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Discipline Hematology Cell Culture Techniques Embryoid Bodies Physiology Animals Blastocyst Cytology Cell Differentiation Cells, Cultured Chimera Coculture Techniques Dendritic Cells Diabetes Mellitus, Type 1 Pathology Embryo Culture Techniques Transplantation Embryonic Stem Cells Gene Expression Profiling Genomic Instability Karyotype Lymphocyte Culture Test, Mixed Mice Mice, Inbred C57bl Mice, Inbred Cba Mice, Inbred Nod Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Hematology

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The origin and efficient derivation of embryonic stem cells in the mouse.

Parameters influencing derivation of embryonic stem cells from murine embryos.

Simple and efficient production of embryonic stem cell-embryo chimeras by coculture.

Replacement of mouse embryonic fibroblasts with bone marrow stromal cells for use in establishing and maintaining embryonic stem cells in mice.

Characterization of a novel embryonic stem cell line from an ICSI-derived blastocyst in the African green monkey.

Optimized protocol for derivation of human embryonic stem cell lines.

Differences in chimera formation and germline transmission between E14 and C2J embryonic stem cells in mice.

Generation of embryonic stem cell lines from immature rabbit ovarian follicles.

Ionizing Radiation Impacts on Cardiac Differentiation of Mouse Embryonic Stem Cells.

Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse.

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Optimization of protocols for derivation of mouse embryonic stem cell lines from refractory strains, including the non obese diabetic mouse.