NDLI: Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

Please wait, while we are loading the content...

Transplantation of mesenchymal stem cells promotes tissue regeneration in a glaucoma model through laser-induced paracrine factor secretion and progenitor cell recruitment.

Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency.

Pim1 serine/threonine kinase regulates the number and functions of murine hematopoietic stem cells.

Developing rods transplanted into the degenerating retina of Crx-knockout mice exhibit neural activity similar to native photoreceptors

Concise review: Current concepts in bone marrow microenvironmental regulation of hematopoietic stem and progenitor cells.

Nicotinamide Overcomes Pluripotency Deficits and Reprogramming $Barriers^{†‡§}$

Year: 2013 Volume: 31 Issue: 5

Year: 2013 Volume: 31 Issue: 4

Year: 2013 Volume: 31 Issue: 3

Year: 2013 Volume: 31 Issue: 2

Year: 2013 Volume: 31 Issue: 1

Year: 2012 Volume: 30

Year: 2011 Volume: 29

Year: 2010 Volume: 28

Year: 2009 Volume: 27

Year: 2008 Volume: 26

Year: 2007 Volume: 25

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sun, Dahui Junger, Wolfgang G. Yuan, Changji Zhang, Wenyan Bao, Yi Qin, Daming Wang, Chengxue Tan, Lei Qi, Baochang Zhu, Dong Zhang, Xizheng Yu, Tiecheng
Description	Author Affiliation: Sun D ( Department of Orthopedics, The First Norman Bethune Hospital of Jilin University, Changchun, People's Republic of China.)
Abstract	Shockwave treatment promotes bone healing of nonunion fractures. In this study, we investigated whether this effect could be due to adenosine 5'-triphosphate (ATP) release-induced differentiation of human mesenchymal stem cells (hMSCs) into osteoprogenitor cells. Cultured bone marrow-derived hMSCs were subjected to shockwave treatment and ATP release was assessed. Osteogenic differentiation and mineralization of hMSCs were evaluated by examining alkaline phosphatase activity, osteocalcin production, and calcium nodule formation. Expression of P2X7 receptors and c-fos and c-jun mRNA was determined with real-time reverse transcription polymerase chain reaction and Western blotting. P2X7-siRNA, apyrase, P2 receptor antagonists, and p38 MAPK inhibitors were used to evaluate the roles of ATP release, P2X7 receptors, and p38 MAPK signaling in shockwave-induced osteogenic hMSCs differentiation. Shockwave treatment released significant amounts (≈ 7 µM) of ATP from hMSCs. Shockwaves and exogenous ATP induced c-fos and c-jun mRNA transcription, p38 MAPK activation, and hMSC differentiation. Removal of ATP with apyrase, targeting of P2X7 receptors with P2X7-siRNA or selective antagonists, or blockade of p38 MAPK with SB203580 prevented osteogenic differentiation of hMSCs. Our findings indicate that shockwaves release cellular ATP that activates P2X7 receptors and downstream signaling events that caused osteogenic differentiation of hMSCs. We conclude that shockwave therapy promotes bone healing through P2X7 receptor signaling, which contributes to hMSC differentiation.
File Format	HTM / HTML
ISSN	10665099
e-ISSN	15494918
DOI	10.1002/stem.1356
Journal	STEM CELLS
Issue Number	6
Volume Number	31
Language	English
Publisher	Wiley
Publisher Date	2013-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Discipline Embryology Adenosine Triphosphate Metabolism Lithotripsy Mesenchymal Stromal Cells Cytology Receptors, Purinergic P2x7 Alkaline Phosphatase Genetics Apyrase Bone Marrow Physiology Calcium Cell Differentiation Cells, Cultured Enzymology Osteocalcin Osteogenesis Proto-oncogene Proteins C-fos Proto-oncogene Proteins C-jun Rna, Messenger Signal Transduction P38 Mitogen-activated Protein Kinases Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

c-fos expression precedes osteogenic differentiation of cartilage cells in vitro

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

Shockwaves increase T-cell proliferation and IL-2 expression through ATP release, P2X7 receptors, and FAK activation.

Expression of c-jun, jun-B, and c-fos Proto-Oncogenes in Human Primary Melanocytes and Metastatic Melanomas

Coordinate occupancy of AP-1 sites in the vitamin D-responsive and CCAAT box elements by Fos-Jun in the osteocalcin gene: model for phenotype suppression of transcription.

Mechanical stretch increases the proliferation while inhibiting the osteogenic differentiation in dental pulp stem cells.

$P2X_{7}$ receptors stimulate AKT phosphorylation in astrocytes

Protein kinases C-beta and C-epsilon link the mast cell high-affinity receptor for IgE to the expression of c-fos and c-jun.

Extracellular Purines Promote the Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells to the Osteogenic and Adipogenic Lineages

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

Similar Documents

c-fos expression precedes osteogenic differentiation of cartilage cells in vitro

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

Shockwaves increase T-cell proliferation and IL-2 expression through ATP release, P2X7 receptors, and FAK activation.

Expression of c-jun, jun-B, and c-fos Proto-Oncogenes in Human Primary Melanocytes and Metastatic Melanomas

Coordinate occupancy of AP-1 sites in the vitamin D-responsive and CCAAT box elements by Fos-Jun in the osteocalcin gene: model for phenotype suppression of transcription.

Mechanical stretch increases the proliferation while inhibiting the osteogenic differentiation in dental pulp stem cells.

$P2X_{7}$ receptors stimulate AKT phosphorylation in astrocytes

Protein kinases C-beta and C-epsilon link the mast cell high-affinity receptor for IgE to the expression of c-fos and c-jun.

Extracellular Purines Promote the Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells to the Osteogenic and Adipogenic Lineages

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors