NDLI: Iron uptake and homeostasis related genes in potato cultivated in vitro under iron deficiency and overload.

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Iron uptake and homeostasis related genes in potato cultivated in vitro under iron deficiency and overload.

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Iron uptake and homeostasis related genes in potato cultivated in vitro under iron deficiency and overload.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Legay, Sylvain Guignard, Cédric Ziebel, Johanna Evers, Danièle
Description	Country affiliation: Luxembourg Author Affiliation: Legay S ( Centre de Recherche Public - Gabriel Lippmann, Department EVA, 41, rue du Brill, L-4422 Belvaux, Luxembourg. legay@lippmann.lu)
Abstract	Potato is one of the most important staple food in the world because it is a good source of vitamin C, vitamin B6 but also an interesting source of minerals including mainly potassium, but also magnesium, phosphorus, manganese, zinc and iron to a lesser extent. The lack of iron constitutes the main form of micronutrient deficiency in the world, namely iron deficiency anemia, which strongly affects pregnant women and children from developing countries. Iron biofortification of major staple food such as potato is thus a crucial issue for populations from these countries. To better understand mechanisms leading to iron accumulation in potato, we followed in an in vitro culture experiment, by qPCR, in the cultivar Désirée, the influence of media iron content on the expression of genes related to iron uptake, transport and homeostasis. As expected, plantlets grown in a low iron medium (1 mg L(-1) FeNaEDTA) displayed a decreased iron content, a strong induction of iron deficiency-related genes and a decreased expression of ferritins. Inversely, plantlets grown in a high iron medium (120 mg L(-1) FeNaEDTA) strongly accumulated iron in roots; however, no significant change in the expression of our set of genes was observed compared to control (40 mg L(-1) FeNaEDTA).
File Format	HTM / HTML
ISSN	09819428
Volume Number	60
e-ISSN	18732690
Journal	Plant Physiology and Biochemistry
Language	English
Publisher	Elsevier
Publisher Date	2012-11-01
Publisher Place	France
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Botany Discipline Biochemistry Gene Expression Regulation, Plant Homeostasis Iron Metabolism Plant Proteins Genetics Solanum Tuberosum Amino Acid Sequence Biological Transport Biomass Cluster Analysis Culture Media Ferritins Analysis Deficiency Magnesium Minerals Molecular Sequence Data Plant Roots Physiology Plant Shoots Plant Stems Rna, Plant Real-time Polymerase Chain Reaction Sequence Alignment Time Factors Zinc Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Plant Science

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