| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Feng, Yanan Zhang, Meng Guo, Qifang Wang, Guokun Gong, Jiangfeng Xu, Ying Wang, Wei |
| Description |
Author Affiliation: Feng Y ( State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.); Zhang M ( State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.); Guo Q ( State Key Laboratory of Crop Biology, College of Agriculture, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.); Wang G ( State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.); Gong J ( State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.); Xu Y ( State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.); Wang W ( State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: wangw@sdau.edu.cn.) |
| Abstract |
Ubiquitin (Ub) is a multifunctional protein that mainly functions to tag proteins for selective degradation by the 26S proteasome. We cloned an Ub gene TaUb2 from wheat (Triticum aestivum L.) previously. To study the function of TaUB2 in chilling stress, sense and antisense Ub transgenic tobacco plants (Nicotiana tabacum L.), as well as wild type (WT) and vector control ß-glucuronidase (T-GUS) plants, were used. Under stress, leaf wilting in sense plants was significantly less than in controls, but more severe in antisense plants. Meanwhile, the net photosynthetic rate (Pn) and the maximal photochemical efficiency of PSII (Fv/Fm) in sense plants were greater than controls, but lower in antisense plants during chilling stress and recovery. Less wilting in sense plants resulted from improved water status, which may be related to the accumulation of proline and solute sugar. Furthermore, as indicated by electrolyte leakage, membrane damage under stress was less in sense plants and more severe in antisense plants than controls. Consistent with electrolyte leakage, the malondialdehyde (MDA) content was less in sense plants, but more in antisense plants compared to controls. Meanwhile, the less accumulation of reactive oxygen species (ROS) and the greater antioxidant enzyme activity in sense plants implied the improved antioxidant competence by the overexpression of monoubiquitin gene Ta-Ub2 from wheat. We suggest that overexpressing Ub is a useful strategy to promote chilling tolerance. The improvement of ROS scavenging may be an important mechanism underlying the role of Ub in promoting plants tolerant to chilling stress. |
| File Format |
HTM / HTML |
| ISSN |
09819428 |
| Volume Number |
75 |
| e-ISSN |
18732690 |
| Journal |
Plant Physiology and Biochemistry |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-02-01 |
| Publisher Place |
France |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Botany Discipline Biochemistry Adaptation, Physiological Genetics Antioxidants Metabolism Cold Temperature Stress, Physiological Tobacco Triticum Ubiquitin Cell Membrane Cloning, Molecular Genes, Plant Glucuronidase Malondialdehyde Oxidative Stress Photosynthesis Photosystem Ii Protein Complex Plant Leaves Plant Proteins Plants, Genetically Modified Proline Reactive Oxygen Species Physiology Water Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Physiology Plant Science |
