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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Balestro, Graciele Carraro Higashi, Bruna Lopes, Sheila Mara Sanches Gonçalves, José Eduardo Vieira, Luiz Gonzaga Esteves de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia |
| Description | Country affiliation: Brazil Author Affiliation: Balestro GC ( Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.); Higashi B ( Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.); Lopes SM ( Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.); Gonçalves JE ( Program of Master in Health Promotion and Program of Master in Clean Technologies, University Center of Maringá, Avenida Guedner, 1610, 87050-900 Maringá, PR, Brazil.); Vieira LG ( Universidade do Oeste Paulista (UNOESTE), Rodovia Raposo Tavares, km 572, 19.067-175 Presidente Prudente, SP, Brazil.); de Oliveira AJ ( Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil.); Gonçalves RA ( Department of Pharmacy, Graduate Program in Pharmaceutical Sciences, State University of Maringá, Avenida Colombo 5790, 87.020-900 Maringá, PR, Brazil. Electronic address: racgoncalves@uem.br.) |
| Abstract | Global interest in sugarcane has increased significantly in recent years because of its economic impact on sustainable energy production. The purpose of the present study was to evaluate changes in the concentrations of total sugars, amino acids, free proline, and total proteins by colorimetric analyses and nuclear magnetic resonance (NMR) to perform a metabolic profiling of a water-soluble fraction of symplastic sap in response to the constitutive expression of a mutant Δ -pyrroline-5-carboxylate synthetase (P5CS) gene from Vigna aconitifolia. However, there was not a significant increase in the free proline content in the sap of transgenic plants compared to the non-transformed control plants. The most noticeable difference between the two genotypes was an almost two-fold increase in the accumulation of sucrose in the stem internodes of P5CS transgenic sugarcane plants. The results presented in this work showed that transgenic sugarcane plants with increased levels of free proline accumulates high soluble sugar content and, therefore, may represent a novel genotype for improving sugarcane cultivars. |
| File Format | HTM / HTML |
| ISSN | 09819428 |
| Journal | Plant Physiology and Biochemistry |
| Volume Number | 113 |
| e-ISSN | 18732690 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-04-01 |
| Publisher Place | France |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Botany Discipline Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Physiology Plant Science |
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